中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2008年
10期
678-681
,共4页
叶梅%夏冰%李冬青%周峰%郭秋莎
葉梅%夏冰%李鼕青%週峰%郭鞦莎
협매%하빙%리동청%주봉%곽추사
胃肿瘤%死亡相关蛋白激酶%甲基化
胃腫瘤%死亡相關蛋白激酶%甲基化
위종류%사망상관단백격매%갑기화
Gastric neoplasms%Death-associated protein kinase%Methylation
目的 研究胃癌组织死亡相关蛋白激酶(DAPK)基因启动子区甲基化对原发性胃癌组织中DAPK mRNA及蛋白表达的影响.方法 采用逆转录(RT)-PCR法检测62例原发性胃癌及癌旁组织DAPK mRNA表达,甲基化特异性PCR(MSP)法检测DAPK启动子区CpG岛甲基化状态,对其中34例胃癌组织甲基化阳性者的DAPK蛋白表达进行Western印迹法检测.结果 胃癌组织中DAPKmRNA和蛋白表达水平明显低于癌旁组织,分别为0.2863±0.2027比0.5736±0.1968、0.2616±0.0913比0.6529±0.1808,差异均有统计学意义(P值均＜0.01).DAPK在胃癌组织和癌旁组织中的甲基化频率分别为54.8%和17.7%,差异有统计学意义(P＜0.01).在胃癌组织中,甲基化组DAPKmRNA表达明显低于非甲基化组(0.1399±0.0835比0.4640±0.1569,P＜0.01).DAPK基因甲基化与胃癌TNM分期显著相关(P=0.04).结论 原发性胃癌组织DAPK mRNA和蛋白表达缺失或低下与其启动子甲基化程度增高显著相关.
目的 研究胃癌組織死亡相關蛋白激酶(DAPK)基因啟動子區甲基化對原髮性胃癌組織中DAPK mRNA及蛋白錶達的影響.方法 採用逆轉錄(RT)-PCR法檢測62例原髮性胃癌及癌徬組織DAPK mRNA錶達,甲基化特異性PCR(MSP)法檢測DAPK啟動子區CpG島甲基化狀態,對其中34例胃癌組織甲基化暘性者的DAPK蛋白錶達進行Western印跡法檢測.結果 胃癌組織中DAPKmRNA和蛋白錶達水平明顯低于癌徬組織,分彆為0.2863±0.2027比0.5736±0.1968、0.2616±0.0913比0.6529±0.1808,差異均有統計學意義(P值均＜0.01).DAPK在胃癌組織和癌徬組織中的甲基化頻率分彆為54.8%和17.7%,差異有統計學意義(P＜0.01).在胃癌組織中,甲基化組DAPKmRNA錶達明顯低于非甲基化組(0.1399±0.0835比0.4640±0.1569,P＜0.01).DAPK基因甲基化與胃癌TNM分期顯著相關(P=0.04).結論 原髮性胃癌組織DAPK mRNA和蛋白錶達缺失或低下與其啟動子甲基化程度增高顯著相關.
목적 연구위암조직사망상관단백격매(DAPK)기인계동자구갑기화대원발성위암조직중DAPK mRNA급단백표체적영향.방법 채용역전록(RT)-PCR법검측62례원발성위암급암방조직DAPK mRNA표체,갑기화특이성PCR(MSP)법검측DAPK계동자구CpG도갑기화상태,대기중34례위암조직갑기화양성자적DAPK단백표체진행Western인적법검측.결과 위암조직중DAPKmRNA화단백표체수평명현저우암방조직,분별위0.2863±0.2027비0.5736±0.1968、0.2616±0.0913비0.6529±0.1808,차이균유통계학의의(P치균＜0.01).DAPK재위암조직화암방조직중적갑기화빈솔분별위54.8%화17.7%,차이유통계학의의(P＜0.01).재위암조직중,갑기화조DAPKmRNA표체명현저우비갑기화조(0.1399±0.0835비0.4640±0.1569,P＜0.01).DAPK기인갑기화여위암TNM분기현저상관(P=0.04).결론 원발성위암조직DAPK mRNA화단백표체결실혹저하여기계동자갑기화정도증고현저상관.
Objective To investigate the regulation effect of promoter methylation of deathassociated protein kinase (DAPK) on mRNA and protein expression of DAPK in tissue of primary gastric cancer (GC). Methods The cancerous and noncancerous samples from 62 patients with GC were determined by RT-PCR for mRNA expression of DAPK. The DAPK promoter methylation was detected by methylation-specific PCR. The protein expression of DAPK in 34 patients with methylation was determined by Western blot. Results mRNA and protein expre.ssions of DAPK in cancerous tissues were reduced significantly compared to noncancerous tissues (0. 2863d±0. 2027 vs 0. 57364±0. 1968,0. 2616±0. 0913 vs 0. 65294±0. 1808, P＜0.01). Methylation frequency of DAPK in cancerous tissues was higher than that in noncancerous tissues (54.8% vs 17.7%, P＜0.01). Furthermore, DAPK mRNA expression was decreased in methylation group compared to unmethylation group (0.1399±0. 0835 vs 0. 46404±0. 1569, P＜0. 01). Moreover, a significant correlation was demonstrated between the TNM stage and DAPK promoter methylation (P = 0. 04). Conclusion Expression of DAPK is down-regulated in cancerous tissues at mRNA and protein levels. Low expression of DAPK is associated with hypermethylation of the promoter of DAPK gene.
