CAJ | 학술논문

为发掘水稻新的耐逆基因,研究植物在应答逆境胁迫时的分子机制,我们采用Affymetrix水稻表达芯片分析了超级稻两优培九母本培矮64S全基因组在多种逆境胁迫下的表达水平,筛选出一批表达水平显著改变的基因.其中基因OsMsD(Oryza sativa L.Multiple Stresses Responsive Gene 9,GenBank登录号为EU276058)的表达量在高温、低温、干旱处理后在多个组织器官、发育时期均有显著提高.实时定量PCR分析其特定时空表达量的结果与基因芯片的结果基本吻合.通过RT-PCR法,得到包含OsMsr9完整ORF(open reading frame)的cDNA克隆.根据生物信息学分析,该ORF编码一个包含168个氨基酸残基的蛋白质,与玉米(Zea mays)中含F-box结构域的全长蛋白有大约58%的相似性,而F-box蛋白可能与植物抗逆性有关.基于上述实验及分析结果,OsMsr9可能是一新的水稻耐逆功能基因.
위발굴수도신적내역기인,연구식물재응답역경협박시적분자궤제,아문채용Affymetrix수도표체심편분석료초급도량우배구모본배왜64S전기인조재다충역경협박하적표체수평,사선출일비표체수평현저개변적기인.기중기인OsMsD(Oryza sativa L.Multiple Stresses Responsive Gene 9,GenBank등록호위EU276058)적표체량재고온、저온、간한처리후재다개조직기관、발육시기균유현저제고.실시정량PCR분석기특정시공표체량적결과여기인심편적결과기본문합.통과RT-PCR법,득도포함OsMsr9완정ORF(open reading frame)적cDNA극륭.근거생물신식학분석,해ORF편마일개포함168개안기산잔기적단백질,여옥미(Zea mays)중함F-box결구역적전장단백유대약58%적상사성,이F-box단백가능여식물항역성유관.기우상술실험급분석결과,OsMsr9가능시일신적수도내역공능기인.
To discover new stress-tolerant genes in rice (Oryza sativa L.) and understand the molecular mechanism(s) underlying stress responses, we analyzed a global genome expression profiling of the indica cuitivar Pei'ai 64S subjected to stresses. We identified a large number of genes highly up-and down-regulated under the stresses. One of these genes, OsMsr9 (Oryza sativa L. Multiple Stresses Responsive Gene 9, GenBank accession: EU276058), was highly induced in leaves and panicles at seedling and booting stages, in response to cold, drought and heat stresses. The expression profile of OsMsr9 obtained by the microarray analysis was confirmed by quantitative real-time PCR analysis of the gene. The two sets of data matched well, suggesting that OsMsr9 is indeed a multiple stress responsive gene in rice. In order to study its function in stress tolerance, the eDNA of the gene was cloned through amplification by RT-PCR. The Bioinformatics analysis indicated that OsMsr9 encodes a protein of 168 amino acids, which shares about 58% similarity with proteins containing F-box domain in Zea mays, which was predicted to be involved in plant stress responses. Based on the above analyses and results obtained, OsMsr9 is probably a novel candidate gene involved in stress tolerance in rice.