CAJ | 학술논문

目的观察Ghrelin激动剂GHRP-2对sepsis诱导产生的急性肺损伤(ALI)大鼠的保护作用.方法 SD大鼠随机分为四组,生理盐水对照组,ALI组,GHRP- 2治疗组和GH对照组,每组12只.通过静脉注射7.5 mg/kg LPS建立ALI模型,ALI模型制备前30 min皮下注射GHRP-2 100 μg/kg或等体积生理盐水.LPS注射后6h处死动物,取动脉血进行血气分析,取肺组织病理学改变,髓过氧化物酶(MPO)水平,肺湿重/肺干重(W/D).取支气管肺泡灌洗液(BALF)观察其中蛋白含量,细胞计数以及炎性介质TNF- α和IL-6的水平.结果脂多糖(LPS)注射后6h,模型大鼠出现明显的氧合障碍,MPO水平,肺W/D比值明显高于生理盐水对照组,而给予GHRP- 2处理的ALI大鼠上述各指标则显著低于ALI组.ALI组BALF中蛋白含量,细胞计数和炎性介质水平明显高于正常对照组,而给予GHRP-2预处理后的ALI大鼠则出现明显降低(P＜0.05).结论静脉注射LPS可成功诱导ALI模型,经GHRP-2预处理后,ALI可显著改善,提示GHRP-2对sepsis诱导的ALI大鼠具有保护作用.
목적 관찰Ghrelin격동제GHRP-2대sepsis유도산생적급성폐손상(ALI)대서적보호작용.방법 SD대서수궤분위사조,생리염수대조조,ALI조,GHRP- 2치료조화GH대조조,매조12지.통과정맥주사7.5 mg/kg LPS건립ALI모형,ALI모형제비전30 min피하주사GHRP-2 100 μg/kg혹등체적생리염수.LPS주사후6h처사동물,취동맥혈진행혈기분석,취폐조직병이학개변,수과양화물매(MPO)수평,폐습중/폐간중(W/D).취지기관폐포관세액(BALF)관찰기중단백함량,세포계수이급염성개질TNF- α화IL-6적수평.결과 지다당(LPS)주사후6h,모형대서출현명현적양합장애,MPO수평,폐W/D비치명현고우생리염수대조조,이급여GHRP- 2처리적ALI대서상술각지표칙현저저우ALI조.ALI조BALF중단백함량,세포계수화염성개질수평명현고우정상대조조,이급여GHRP-2예처리후적ALI대서칙출현명현강저(P＜0.05).결론 정맥주사LPS가성공유도ALI모형,경GHRP-2예처리후,ALI가현저개선,제시GHRP-2대sepsis유도적ALI대서구유보호작용.
Objective To investigate the effects of GHRP-2 on sepsis induced acute lung injury (ALI) rats.Methods SD rats weighting 200-250 g were randomly divided into four groups ( n =12 per group):saline control group,ALI group,GHRP-2/ALI group,GHRP 2 control group.ALI was induced by administering 7.5 mg/kg lipopolysaccharide (LPS) via vein.The rats were subcutaneously treated with 100 μg/kg GHRP-2 30 min before LPS administration.Rats were sacrificed at 6 h after LPS administration,blood were obtained to observe blood gas,lung tissue were used to examined lung pathology changes,lung edema and myeloperoxidase level.Moreover,protein content,cell count and inflammatory cytokines TNF-α and IL-6 levels were detected in BALF.Results 6 h after LPS administration,rats appeared obvious decreased oxygenation,meanwhile,lung injury score,MPO level and W/D ratio were significantly enhanced in ALI rats compared with saline group ( P ＜0.05),GHRP-2 pretreatment obviously decreased these lung iniury indexes.In BALF,ALI rats has obviously protein level,cell count and inflammatory cytokines level,however,GHRP- 2 pretreatment significantly reduced these indexes in BALF.No obvious difference in saline control group and GHRP-2 control group.Conclusions GHRP- 2 could decrease the inflammation response and prevent the development of ALI which caused by LPS which suggest a potential therapy agent for ALI.