CAJ | 학술논문

目的探讨免疫磁珠(IMB)检测方法用于结直肠癌腹腔内脱落癌细胞的检测价值.方法收集80例结直肠癌患者术中的腹腔冲洗液,分别进行IMB及常规腹腔冲洗液细胞学检查(PLC),检测腹腔内脱落癌细胞,并以10例妇产科子宫肌瘤手术患者腹腔冲洗液标本作为对照组.结果 PLC检测阳性率为8.8％(7/80),IMB检测阳性率为28.8％(23/80),PLC检测阳性的病例,IMB均为阳性.IMB检出率明显高于PLC(x2=10.503,P=0.001).对照组两种方法检测均为阴性结果.结论 IMB对于结直肠癌腹腔脱落癌细胞检出率较PLC有较大提高,为腹腔癌细胞微转移的发现提供了一个切实可行的有效途径.
목적 탐토면역자주(IMB)검측방법용우결직장암복강내탈락암세포적검측개치.방법 수집80례결직장암환자술중적복강충세액,분별진행IMB급상규복강충세액세포학검사(PLC),검측복강내탈락암세포,병이10례부산과자궁기류수술환자복강충세액표본작위대조조.결과 PLC검측양성솔위8.8％(7/80),IMB검측양성솔위28.8％(23/80),PLC검측양성적병례,IMB균위양성.IMB검출솔명현고우PLC(x2=10.503,P=0.001).대조조량충방법검측균위음성결과.결론 IMB대우결직장암복강탈락암세포검출솔교PLC유교대제고,위복강암세포미전이적발현제공료일개절실가행적유효도경.
Objective To explore the value of immuomagnetic beads(IMB) technique for detection of intraperitoneal free cancer cells from colorectal cancer.Methods Peritoneal lavage fluid was obtained from 80 patients with colorectal cancer during laparotomy.Peritoneal lavage cytology (PLC) and IMB were used to detect free cancer cells in peritoneal lavage fluid.10 patients with hysteromyoma during laparotomy were enrolled into the control group.Results The positive rate of PLC was 8.8％ (7/80),the positive rate of IMB was 28.8％ (23/80).The positive case after useing PLC detect,IMB detect also was positive.The detected samples of control group were negative by these two methods.IMB was superior to PLC ( x2 =10.503,P =0.001 ).Conclusion IMB was more sensitive and specific than PLC,which could provide a effective method for finding intraperitoneal free cancer cells.