中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
10期
903-907
,共5页
刘毓和%吴新民%应舜伟%杜敏逸%陈揭晓
劉毓和%吳新民%應舜偉%杜敏逸%陳揭曉
류육화%오신민%응순위%두민일%진게효
麻醉药%吸入%认知障碍%凋亡蛋白酶活化因子1%半胱氨酸天冬氨酸蛋白酶9%老年人
痳醉藥%吸入%認知障礙%凋亡蛋白酶活化因子1%半胱氨痠天鼕氨痠蛋白酶9%老年人
마취약%흡입%인지장애%조망단백매활화인자1%반광안산천동안산단백매9%노년인
Anesthetics%inhalation%Cognition disorders%Apoptotic protease-activating factor 1%Caspase 9%Aged
目的 探讨反复吸入七氟醚对老龄大鼠海马组织凋亡蛋白酶活化因子-1(Apaf-1)及半胱氨酸天冬氨酸蛋白酶-9(Caspase-9)mRNA表达的影响.方法 健康Wistar大鼠30只,月龄22月,体重380～770 g,雌雄各半,随机分为4组:对照组(n=8,Ⅰ组)、2%七氟醚组(n=8,Ⅱ组)、3%七氟醚组(n=8,Ⅲ组)及颈总动脉结扎+2%七氟醚组(n=6,IV组).Ⅰ组不吸入七氟醚,Ⅱ组及Ⅲ组分别吸入2%、3%七氟醚,100 min/d,连续5 d.Ⅳ组结扎左侧颈总动脉,1周后吸入2%七氟醚,100 min/d,连续5 d.停止吸入七氟醚后第1天开始采用Morris水迷宫实验测定大鼠认知功能,4次/d,连续测定5 d(T_(1～5)),记录潜伏期、平台象限活动时间与总游泳时间的百分比(Tp/T)及撤去平台后2 min内的游泳轨迹.水迷宫实验结束后采用RT-PCR法测定海马组织Apaf-1、Caspase-9 mRNA的表达水平.结果 与T_1时比较,Ⅰ组T_(3～5)、Ⅱ组及Ⅲ组T_(2～5)时、Ⅳ组T_(4,5)时潜伏期缩短(P<0.05或0.01);与Ⅰ组比较,Ⅲ组T_1时、Ⅳ组T_(1～5),时潜伏期延长,Ⅲ组及Ⅳ组Tp/T降低,Ⅳ组Caspase-9 mRNA表达上调(P<0.05或0.01).撤去平台后,Ⅱ组大鼠游泳轨迹明显集中在平台象限(Ⅳ象限),Ⅲ组和Ⅳ组大鼠游泳轨迹明显散乱.结论 反复吸入七氟醚可导致老龄大鼠认知功能障碍,其机制与海马组织Apaf-1及Caspase-9 mRNA的表达无关.
目的 探討反複吸入七氟醚對老齡大鼠海馬組織凋亡蛋白酶活化因子-1(Apaf-1)及半胱氨痠天鼕氨痠蛋白酶-9(Caspase-9)mRNA錶達的影響.方法 健康Wistar大鼠30隻,月齡22月,體重380～770 g,雌雄各半,隨機分為4組:對照組(n=8,Ⅰ組)、2%七氟醚組(n=8,Ⅱ組)、3%七氟醚組(n=8,Ⅲ組)及頸總動脈結扎+2%七氟醚組(n=6,IV組).Ⅰ組不吸入七氟醚,Ⅱ組及Ⅲ組分彆吸入2%、3%七氟醚,100 min/d,連續5 d.Ⅳ組結扎左側頸總動脈,1週後吸入2%七氟醚,100 min/d,連續5 d.停止吸入七氟醚後第1天開始採用Morris水迷宮實驗測定大鼠認知功能,4次/d,連續測定5 d(T_(1～5)),記錄潛伏期、平檯象限活動時間與總遊泳時間的百分比(Tp/T)及撤去平檯後2 min內的遊泳軌跡.水迷宮實驗結束後採用RT-PCR法測定海馬組織Apaf-1、Caspase-9 mRNA的錶達水平.結果 與T_1時比較,Ⅰ組T_(3～5)、Ⅱ組及Ⅲ組T_(2～5)時、Ⅳ組T_(4,5)時潛伏期縮短(P<0.05或0.01);與Ⅰ組比較,Ⅲ組T_1時、Ⅳ組T_(1～5),時潛伏期延長,Ⅲ組及Ⅳ組Tp/T降低,Ⅳ組Caspase-9 mRNA錶達上調(P<0.05或0.01).撤去平檯後,Ⅱ組大鼠遊泳軌跡明顯集中在平檯象限(Ⅳ象限),Ⅲ組和Ⅳ組大鼠遊泳軌跡明顯散亂.結論 反複吸入七氟醚可導緻老齡大鼠認知功能障礙,其機製與海馬組織Apaf-1及Caspase-9 mRNA的錶達無關.
목적 탐토반복흡입칠불미대노령대서해마조직조망단백매활화인자-1(Apaf-1)급반광안산천동안산단백매-9(Caspase-9)mRNA표체적영향.방법 건강Wistar대서30지,월령22월,체중380～770 g,자웅각반,수궤분위4조:대조조(n=8,Ⅰ조)、2%칠불미조(n=8,Ⅱ조)、3%칠불미조(n=8,Ⅲ조)급경총동맥결찰+2%칠불미조(n=6,IV조).Ⅰ조불흡입칠불미,Ⅱ조급Ⅲ조분별흡입2%、3%칠불미,100 min/d,련속5 d.Ⅳ조결찰좌측경총동맥,1주후흡입2%칠불미,100 min/d,련속5 d.정지흡입칠불미후제1천개시채용Morris수미궁실험측정대서인지공능,4차/d,련속측정5 d(T_(1～5)),기록잠복기、평태상한활동시간여총유영시간적백분비(Tp/T)급철거평태후2 min내적유영궤적.수미궁실험결속후채용RT-PCR법측정해마조직Apaf-1、Caspase-9 mRNA적표체수평.결과 여T_1시비교,Ⅰ조T_(3～5)、Ⅱ조급Ⅲ조T_(2～5)시、Ⅳ조T_(4,5)시잠복기축단(P<0.05혹0.01);여Ⅰ조비교,Ⅲ조T_1시、Ⅳ조T_(1～5),시잠복기연장,Ⅲ조급Ⅳ조Tp/T강저,Ⅳ조Caspase-9 mRNA표체상조(P<0.05혹0.01).철거평태후,Ⅱ조대서유영궤적명현집중재평태상한(Ⅳ상한),Ⅲ조화Ⅳ조대서유영궤적명현산란.결론 반복흡입칠불미가도치노령대서인지공능장애,기궤제여해마조직Apaf-1급Caspase-9 mRNA적표체무관.
Objective To investigate the effects of repeated inhalation of sevoflurane on the expression of apoptotic protease-activating factor-1 (Apaf-1) and Caspase-9 mRNA in hippocampus in elderly rats. Methods Thirty Wistar rats (15 males, 15 females) aged 22 months weighing 380-770 g were randomly divided into 4 groups: control group (n=8, group Ⅰ), 2% sevoflurane (n=8, group Ⅱ), 3 % sevoflurane (n=8, group Ⅲ) and ligation of left common carotid artery + 2% sevoflurane group (n=6, group Ⅳ). Group Ⅰ inhaled oxygen (2.5 L/min) and nitrogen (6 L/min). Group Ⅱ and Ⅲ inhaled 2% and 3% sevoflurane in oxygen (2.5 L/min) and nitrogen (6 L/min) 100 min/d for 5 consecutive days respectively. In group Ⅳ, left common carotid artery was ligated and 2% sevoflurane inhaled 100 min/d for 5 consecutive days one week later. Cognitive function was detected 4 times a day for 5 consecutive days using Morris water-maze (MWM) test after the last inhalation (T_(1-5)). The latency, percentage of swimming time in the platform quadrant (T_P/T) and swimming traces within 2 min after the removal of platform were recorded. The rats were sacrificed immediately after the last test and hippocampus immediately removed for determination of Apaf-1 and caspase-9 mRNA expression using RT-PCR. Results The latency at T_(3-5) in group I , T_(2-5) in group Ⅱ and Ⅲ and T_(4-5) in group Ⅳ was significantly shorter than that at T_1 (P < 0.05 or 0.01). The latency was significantly prolonged at T_1 in group Ⅲ and at T_(1-5) in group Ⅳ, Tp/T significantly decreased in group Ⅲ and Ⅳ and Caspase-9 mRNA expression up-regulated in group Ⅳ compared with group Ⅰ (P<0.05 or 0.01). After the platform was removed, the swimming traces were located in platform quadrant (quadrant Ⅳ) in group Ⅱ, while obviously in disorder in group Ⅲ and Ⅳ. Conclusion Repeated inhalation of sevoflurane can result in cognitive dysfunction and the mechanism is not related to Apaf-1 and Caspase-9 mRNA expression.
