中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2009年
3期
145-149
,共5页
张逸仙%刘楠%杜厚伟%陈荣华%王杰华%林克旭
張逸仙%劉楠%杜厚偉%陳榮華%王傑華%林剋旭
장일선%류남%두후위%진영화%왕걸화%림극욱
脑缺血%康复训练%胰岛素样生长因子-1%胰岛素样生长因子-1受体
腦缺血%康複訓練%胰島素樣生長因子-1%胰島素樣生長因子-1受體
뇌결혈%강복훈련%이도소양생장인자-1%이도소양생장인자-1수체
Cerebral ischemia%Rehabititative training%Insulin-like growth factor-1%Insulin-like growth factor-1 receptor
目的 探讨跑台运动训练对局灶性脑缺血大鼠神经功能恢复和脑缺血组织中胰岛素样生长因子-1(IGF-1)及其受体(IGF-1R)表达的影响.方法 42只清洁级成年雄性SD大鼠,随机分为假手术组(n=6)、模型组(n=18)和运动组(n=18).模型组、运动组大鼠采用改良的Longa线栓法制备大脑中动脉闭塞(MCAO)脑缺血模型.运动组于造模成功后24 h采用跑台训练器进行运动训练,每周6 d.共4周;其余2组则置于普通笼内饲养,期间可自由活动、进食.采用修正的神经行为学评分方法评价模型组和运动组大鼠MCAO术后第7,14,28天的神经功能,并断头取脑,采用逆转录多聚酶链式反应(RT-PCR)法和免疫组织化学方法检测脑缺血组织中IGF-1、IGF-1R的表达.结果 运动组大鼠术后14,28 d神经功能评分均明显优于模型组(P＜0.01和P＜0.05);运动组大鼠术后7,14,28 d,IGF-1、IGF-1R表达较模型组明显增强(P＜0.05或P＜0.01).结论 运动训练可促进脑缺血大鼠神经功能恢复,其机制可能与上调脑缺血组织中IGF-1、IGF-1R表达有关.
目的 探討跑檯運動訓練對跼竈性腦缺血大鼠神經功能恢複和腦缺血組織中胰島素樣生長因子-1(IGF-1)及其受體(IGF-1R)錶達的影響.方法 42隻清潔級成年雄性SD大鼠,隨機分為假手術組(n=6)、模型組(n=18)和運動組(n=18).模型組、運動組大鼠採用改良的Longa線栓法製備大腦中動脈閉塞(MCAO)腦缺血模型.運動組于造模成功後24 h採用跑檯訓練器進行運動訓練,每週6 d.共4週;其餘2組則置于普通籠內飼養,期間可自由活動、進食.採用脩正的神經行為學評分方法評價模型組和運動組大鼠MCAO術後第7,14,28天的神經功能,併斷頭取腦,採用逆轉錄多聚酶鏈式反應(RT-PCR)法和免疫組織化學方法檢測腦缺血組織中IGF-1、IGF-1R的錶達.結果 運動組大鼠術後14,28 d神經功能評分均明顯優于模型組(P＜0.01和P＜0.05);運動組大鼠術後7,14,28 d,IGF-1、IGF-1R錶達較模型組明顯增彊(P＜0.05或P＜0.01).結論 運動訓練可促進腦缺血大鼠神經功能恢複,其機製可能與上調腦缺血組織中IGF-1、IGF-1R錶達有關.
목적 탐토포태운동훈련대국조성뇌결혈대서신경공능회복화뇌결혈조직중이도소양생장인자-1(IGF-1)급기수체(IGF-1R)표체적영향.방법 42지청길급성년웅성SD대서,수궤분위가수술조(n=6)、모형조(n=18)화운동조(n=18).모형조、운동조대서채용개량적Longa선전법제비대뇌중동맥폐새(MCAO)뇌결혈모형.운동조우조모성공후24 h채용포태훈련기진행운동훈련,매주6 d.공4주;기여2조칙치우보통롱내사양,기간가자유활동、진식.채용수정적신경행위학평분방법평개모형조화운동조대서MCAO술후제7,14,28천적신경공능,병단두취뇌,채용역전록다취매련식반응(RT-PCR)법화면역조직화학방법검측뇌결혈조직중IGF-1、IGF-1R적표체.결과 운동조대서술후14,28 d신경공능평분균명현우우모형조(P＜0.01화P＜0.05);운동조대서술후7,14,28 d,IGF-1、IGF-1R표체교모형조명현증강(P＜0.05혹P＜0.01).결론 운동훈련가촉진뇌결혈대서신경공능회복,기궤제가능여상조뇌결혈조직중IGF-1、IGF-1R표체유관.
Objective To study the effects of treadmill training on the recovery of neurological function and the expression of IGF-1/IGF-1Rin the brain poat focal cerebral ischemia.Metheods Forty-two male,adult Sprague-Dawley rats were randomly divided into a sham group(n=6),a model group(n=18)and a treadmill exercises group(n=18).Focal cerebral ischemia was induced in the latter two groups by right middle cerebral artery occlusion(MCAO)using a modified Longa's metheod.The rats in the treadmill exercises group were given treadmill training 6 days a week for 4weeks beginning 24 h after the MCAO. Rats in the sham group and model groups were reared in standard cages and could move frddly. Before being sacrificed at the 7th 14th and 28th day after the MCAO,the rats' neurological function were tested giving modified neurological weverity scores (mNSS). The expression of IGF-1/IGF-1R was detected using the reverse transcriptsae polymersae chain reaction and immunohistochemical methods.Results Neurological runction in the exercises group at the 14th and 28th day was better than in the control group,and IGF-1 and IGF-1Rexpression were significcantly upregulated on the 7th,14th and 28th day after the MCAO.Conclusion Treadmill training can improve neurological function by upregulating the expression of IGF-1 and IGF-1R in the ischemic brain, at least in rats.
