CAJ | 학술논문

[目的]观察持续频闪光刺激对发育敏感期豚鼠视网膜组织及功能的影响.[方法]24只出生日龄14 d的豚鼠随机分为频闪组和对照组,每组均为12只.频闪组使用频闪调光器以0.5 Hz频率等时交替频闪,照度波动0～500 Lux；对照组给予500 Lux照明.2组均使用500 nm波长发光二极管,控制光照时间为昼夜12h轮替.实验第12周所有豚鼠行跟底彩色照相和闪光视网膜电图(F-ERG)检查.检查结束后摘除眼球,测量眼球水平径、垂直径及前后径3条径线,光学显微镜和透射电子显微镜观察眼球后极部结构改变.[结果]与对照组比较,频闪组豚鼠眼底漆裂纹样改变更为明显,ERG a波潜伏期延长;眼球水平径、垂直径及前后径分别较对照组增加(0.89±0.30)、(0.69±0.20)、(0.96±0.30)mm,差异有统计学意义(t=12.7、11.9、15.8,P＜0.05).光学显微镜观察发现,频闪组豚鼠巩膜纤维出现扩张；透射电子镜显微镜观察发现,视网膜感光细胞层外段排列稀疏紊乱并可见大量脱落外节盘膜.[结论]持续频闪光刺激会诱导豚鼠眼球产生过度发育,并会影响视网膜结构与传导功能.
[목적]관찰지속빈섬광자격대발육민감기돈서시망막조직급공능적영향.[방법]24지출생일령14 d적돈서수궤분위빈섬조화대조조,매조균위12지.빈섬조사용빈섬조광기이0.5 Hz빈솔등시교체빈섬,조도파동0～500 Lux；대조조급여500 Lux조명.2조균사용500 nm파장발광이겁관,공제광조시간위주야12h륜체.실험제12주소유돈서행근저채색조상화섬광시망막전도(F-ERG)검사.검사결속후적제안구,측량안구수평경、수직경급전후경3조경선,광학현미경화투사전자현미경관찰안구후겁부결구개변.[결과]여대조조비교,빈섬조돈서안저칠렬문양개변경위명현,ERG a파잠복기연장;안구수평경、수직경급전후경분별교대조조증가(0.89±0.30)、(0.69±0.20)、(0.96±0.30)mm,차이유통계학의의(t=12.7、11.9、15.8,P＜0.05).광학현미경관찰발현,빈섬조돈서공막섬유출현확장；투사전자경현미경관찰발현,시망막감광세포층외단배렬희소문란병가견대량탈락외절반막.[결론]지속빈섬광자격회유도돈서안구산생과도발육,병회영향시망막결구여전도공능.
[Objective] To observe the effect of persistent flickering stimulus on the structure and function of retina in guinea pigs during a developmentally sensitive period.[Methods] Twenty-four twoweek-old guinea pigs were randomly divided into flicker light (FL) group and control group,with 12 guinea pigs in each group.Animals in FL group were raised under 500 Lux illumination with a duty diurnal cycle of 50％ at a flash rate of 0.5 Hz.Animals in control group were reared under steady 500 Lux illumination.Light emitting diode (LED) lamps were used for lighting under a 12-hour light/12-hour dark cycle.After the collection of fundus photographs and electroretinograms recorded at week 12,eyeballs were taken out,three dimensions were measured,and histopathological changes were examined.[Results] Compared to control group,tessellated fundus in FL group appeared more prevalent; implicit time of “a” waves were prolonged in electroretinogram; the eyeballs were increased in horizontal,vertical,axial dimensions by (0.89±0.30),(0.69±0.20) and (0.96±0.30) mm respectively,the differences between two groups were statistically significant (t =12.7,11.9,15.8; P ＜ 0.05 ).The gap of sclera collagen fiber was slightly widened.The photoreceptor layer was more likely to develop a disordered outer segment,which contained deciduous disc membranes.[Conclusion] Persistent flickering stimulus is attended by development of excessive ocular enlargement,which could affect the retinal structure and function of photoreceptors.