华中科技大学学报(医学英德文版)
華中科技大學學報(醫學英德文版)
화중과기대학학보(의학영덕문판)
JOURNAL OF TONGJI MEDICAL UNIVERSITY
2002年
4期
279-280,287
,共3页
林先明%李真%胡本容%夏国瑾%姚伟星%向继洲
林先明%李真%鬍本容%夏國瑾%姚偉星%嚮繼洲
림선명%리진%호본용%하국근%요위성%향계주
arecoline%patch elamp%calcium channel%laser scanning confocal microscope%cytoso-lic Ca2+ level
Summary: The effects of Arecoline (Are) on calcium mobilization were investigated. In isolatedsingle ventricular myocyte of guinea pig, patch clamp whole cell recording techniques were used torecord the current of L-type calcium channel and cytosolic Ca2+ level ([Ca2+]i) labeled with fluo-rescence probe Fluo-3/AM was measured under a laser scanning confocal microscope. Results re-vealed that Are (3-100 μmol/L) could inhibit L-type calcium current in a concentration-depen-dent manner and the value of IC50 was 33. 73μmol/L (n= 5). In the absence of extracellular calci-um, the resting levels of [Ca2+]i was not affected by Are (n=6, P＞0. 05), but pretreatmentwith Are (30 μmol/L) could significantly inhibit the [Ca2+]i elevation induced by caffeine (10mmol/L, n = 6, P ＜ 0. 01). It was concluded that Are could inhibit not only calcium influxthrough L-type calcium channel but also calcium release from sarcoplasmic reticulum.
