遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2001年
1期
83-94
,共12页
作图%分子标记%F2群体%Fisher精确检测
作圖%分子標記%F2群體%Fisher精確檢測
작도%분자표기%F2군체%Fisher정학검측
作者从数学上导出了基因作图的三点自交方法。这一方法同三点测交法一样能提供各种作图信息，但不需要选育三隐性纯合基因亲本或品系，因而能大大提高作图功效。从理论上证明，该方法也适合于小群体作图分子标记连锁图谱。同时用Fisher单一观察信息（即F信息）量证明，三点自交法是一种有效的作图方法。应用MAPMAKER程序中所提供的老鼠F2群体中333个个体的12个RFLP标记位点中前6个位点的数据对三点自交法的作图功能进行了检测。结果表明，三点自交法在位点间的连锁关系检测，连锁群中位点的排序和图距计算具有与MAPMAKER程序一样的功能，而且还提供了位点间的交叉干涉和位点的相引或相斥构型等信息以及紧密位点间发生负干涉作用的证据。
作者從數學上導齣瞭基因作圖的三點自交方法。這一方法同三點測交法一樣能提供各種作圖信息，但不需要選育三隱性純閤基因親本或品繫，因而能大大提高作圖功效。從理論上證明，該方法也適閤于小群體作圖分子標記連鎖圖譜。同時用Fisher單一觀察信息（即F信息）量證明，三點自交法是一種有效的作圖方法。應用MAPMAKER程序中所提供的老鼠F2群體中333箇箇體的12箇RFLP標記位點中前6箇位點的數據對三點自交法的作圖功能進行瞭檢測。結果錶明，三點自交法在位點間的連鎖關繫檢測，連鎖群中位點的排序和圖距計算具有與MAPMAKER程序一樣的功能，而且還提供瞭位點間的交扠榦涉和位點的相引或相斥構型等信息以及緊密位點間髮生負榦涉作用的證據。
작자종수학상도출료기인작도적삼점자교방법。저일방법동삼점측교법일양능제공각충작도신식，단불수요선육삼은성순합기인친본혹품계，인이능대대제고작도공효。종이론상증명，해방법야괄합우소군체작도분자표기련쇄도보。동시용Fisher단일관찰신식（즉F신식）량증명，삼점자교법시일충유효적작도방법。응용MAPMAKER정서중소제공적로서F2군체중333개개체적12개RFLP표기위점중전6개위점적수거대삼점자교법적작도공능진행료검측。결과표명，삼점자교법재위점간적련쇄관계검측，련쇄군중위점적배서화도거계산구유여MAPMAKER정서일양적공능，이차환제공료위점간적교차간섭화위점적상인혹상척구형등신식이급긴밀위점간발생부간섭작용적증거。
The method for constructing linkage maps has almost been so farthe three-point testcross in higher organisms. This method is, however, considerably limited to a requirement of a parent or line with three recessive genes that can be obtained by only cross breeding. In this paper, the three-point selfcross method for mapping was proposed. This method can also provide us with informations of mapping as the same as the three杙oint testcross method. However, the three杙oint selfcross method does not have any limitation of requiring a parent with three recessive genes. It was theoretically proved that this method could be used to map molecular marker loci in small sample of F2 population. Exactly to test both the methods for mapping, Fisher information content was applied to prove that the three-point selfcross method is powerful for mapping. Further to test this method, data of the first 6 loci were chosen from 12 RFLP loci detected in 333 F2 individuals in SAMPLE. RAW file in MAPMAKER／EXP version 3.0 that Lander et al. (1987) provided. The result showed that, like MAPMAKER program, the three-point selfcross method is powerful to detect linkage relationship among loci, to order them on linkage groups and to calculate distances between nearest neighbor loci. Besides, this method could provide us with the other information of map such as the positive or negative interference and couple or repulsion configuration.
