CAJ | 학술논문

目的:研究选择性磷酸二酯酶3抑制剂奥普力农对卵蛋白致敏和激发豚鼠咳嗽反应的影响.方法:卵蛋白致敏豚鼠40只,随机分成对照组、激发组、奥普力农组和氨茶碱组,每组10只.1%卵蛋白或生理盐水雾化吸入激发后2 h,腹腔内分别注射生理盐水、25 mg/kg的奥普力农或氨茶碱.激发后24h,奥普力农组和氨茶碱组豚鼠腹腔依次注射2.5 mg/kg和25 mg/kg的奥普力农或氨茶碱,测量吸入辣椒素溶液诱导的咳嗽反应和对乙酰甲胆碱溶液的气道反应性(PC150),分析支气管肺泡灌洗液中的细胞数和细胞成分.结果:对照组和激发组豚鼠的咳嗽频率分别为(5±1)times/3 min和(24±3)times/3 min(P＜0.05),PC150分别为(659±57)mg/L和(238±67)mg/L(P＜0.05).25 mg/kg的奥普力农能明显抑制这些改变,咳嗽频率和PC150分别为(15±2)times/3 min和(580±45)mg/L(P＜0.05),作用强于同剂量氨茶碱的(18±2)times/3 min和(438±52)mg/L(P＜0.05).而奥普力农组豚鼠支气管肺泡灌洗液中细胞总数和嗜酸粒细胞比例和激发组比较无显著差异(P＞0.05).结论:选择性磷酸二酯酶3抑制剂通过支气管舒张作用减轻嗜酸粒细胞性气道炎症所致咳嗽.
목적:연구선택성린산이지매3억제제오보력농대란단백치민화격발돈서해수반응적영향.방법:란단백치민돈서40지,수궤분성대조조、격발조、오보력농조화안다감조,매조10지.1%란단백혹생리염수무화흡입격발후2 h,복강내분별주사생리염수、25 mg/kg적오보력농혹안다감.격발후24h,오보력농조화안다감조돈서복강의차주사2.5 mg/kg화25 mg/kg적오보력농혹안다감,측량흡입랄초소용액유도적해수반응화대을선갑담감용액적기도반응성(PC150),분석지기관폐포관세액중적세포수화세포성분.결과:대조조화격발조돈서적해수빈솔분별위(5±1)times/3 min화(24±3)times/3 min(P＜0.05),PC150분별위(659±57)mg/L화(238±67)mg/L(P＜0.05).25 mg/kg적오보력농능명현억제저사개변,해수빈솔화PC150분별위(15±2)times/3 min화(580±45)mg/L(P＜0.05),작용강우동제량안다감적(18±2)times/3 min화(438±52)mg/L(P＜0.05).이오보력농조돈서지기관폐포관세액중세포총수화기산립세포비례화격발조비교무현저차이(P＞0.05).결론:선택성린산이지매3억제제통과지기관서장작용감경기산립세포성기도염증소치해수.
AIM: To investigate the effects of selective phosphodiesterase 3 inhibitor olprinone on cough response in guinea pigs sensitized and challenged with ovalbumin. METHODS: Forty sensitized guinea pigs were randomly divided into control ( n = 10), challenged ( n = 10), olprinone ( n = 10) and aminophylline group ( n = 10 ). Two hours after challenged with the aerosol of 1% ovalbumin or saline, animals were intraperitoneally injected either with saline,25 mg/kg of olprinone or 25 mg/kg aminophylline. At 24 h, the injection was repeated with 2. 5 mg/kg and 25 mg/kg olprinone or 2. 5 mg/kg and 25 mg/kg aminophylline respectively in olprinone and aminophylline group, cough response to inhaled capsaicin and airway responsiveness to methacholine (PC150) were measured. Then, total cell number and differential counts were analyzed in bronchoalveolar lavage fluid. RESULTS: The cough frequency was (5 ± 2) times/3 min in control group and (24 ± 3 ) times/3 min in challenged group ( P ＜ 0. 05 ), while PC150 was (659 + 57 ) mg/L in control group and (238 + 67 ) mg/L inchallenged group ( P ＜ 0. 05 ). 25 mg/kg olprinone significantly inhibited the augmented cough response and airway hyperresponsiveness, the cough frequency and PC150 were (15 ±2) times/3 min and (580 ±45) mg/L (P ＜ 0. 05 ), which differed significantly from (18 ± 2) times/3 min and (438± 52) mg/L in aminophilline group (P ＜ 0. 05). However, olprinone failed to reverse the elevated total cell number and percentage of eosinophils in bronchoalveolar lavage fluid from guinea pigs challenged with ovalbumin (P ＞ 0. 05 ). CONCLUSION: Phosphodiesterase 3 inhibitor attenuates cough response associated with eosinophilic airway inflammation by bronchodilatory effect.