CAJ | 학술논문

该文利用23个微卫星标记对103尾大黄鱼繁育亲本进行遗传多样性检测和亲缘关系重建分析,并构建繁育体系指导大黄鱼配组.遗传多样性检测显示,103尾亲鱼任23个座位共获得等位基因数134个,平均5.82个,总平均观察杂合度0.5993,表明该繁育群体尚保持一定水平的遗传多样性.采用似然率和组合优化法统计模型重建的同胞关系不尽相同,但结果均证实了这些繁育亲本亲缘关系十分相近.配组比对分析结果显示,两种方法的配组方案一致性高达85%,最终选择组合优化法的分组方案指导大黄鱼配组繁育.
해문이용23개미위성표기대103미대황어번육친본진행유전다양성검측화친연관계중건분석,병구건번육체계지도대황어배조.유전다양성검측현시,103미친어임23개좌위공획득등위기인수134개,평균5.82개,총평균관찰잡합도0.5993,표명해번육군체상보지일정수평적유전다양성.채용사연솔화조합우화법통계모형중건적동포관계불진상동,단결과균증실료저사번육친본친연관계십분상근.배조비대분석결과현시,량충방법적배조방안일치성고체85%,최종선택조합우화법적분조방안지도대황어배조번육.
Present broodstocks of large yellow croaker are borne from extremely small numbers of base population.Thus, it is necessary to analyze kinship of broodstocks in order to avoid inbreeding that will bring out the reduction of individual survival and growth. This paper reports kinship reconstruction and genetic diversity in 103 broodstocks of large yellow croaker by utilizing 23 microsatellite markers. Genetic diversities of 103 croakers at 23 loci pronounce that there are 134 alleles in total and an average of 5.82, and the observed average heterozygosity of 0.5993, demonstrating that these broodstocks still maintain genetic variability to some extent. The results of sibling groups reconstructed are not identical using two methods of Likelihood and 2-allele recombinatorial optimization. However, the evidence of close relationship between broodstocks is confirmed. The mating combinations are compared between these two methods, as a result of 85% identity and a final selection of 2-allele method. This study aims at finding a better way to avoid inbreeding occurred in broodstocks, and facilitating the aquaculture market of large yellow croaker, meanwhile, offering methods and statistical models to artificial propagation in other marine fish species.