CAJ | 학술논문

DNA计箅作为前沿科学研究的重点和热点.已经从简单发展为复杂,从理论转化为应用.在这一过程中,反应速度快、变化灵敏的荧光标记技术发挥了重要的作用.文中围绕DNA计算和荧光标记技术两个方面进行说明.一方面,对近年来DNA计算中荧光技术的应用进行了总结:(1)荧光标记的表面计算;(2)与某些切技术相结合的荧光检测;(3)与DNA链置换相结合的荧光技术;(4)与基因沉默技术相结合的荧光DNA逻辑门;(5)与DNA自组装立体结构相结合的荧光技术;(6)与DNA变构相结合的荧光技术.另一方面,介绍了几种近年来发展起来的新型荧光技术:(1)荧光信号识别放大技术;(2)与磁珠技术相结合的荧光技术;(3)与PH值变化相结合的DNA荧光技术;(4)与miRNAs检测相结合的荧光技术.在今后的研究中,只有将这两者紧密结合,才能发挥DNA计算天然的优势.
DNA계폐작위전연과학연구적중점화열점.이경종간단발전위복잡,종이론전화위응용.재저일과정중,반응속도쾌、변화령민적형광표기기술발휘료중요적작용.문중위요DNA계산화형광표기기술량개방면진행설명.일방면,대근년래DNA계산중형광기술적응용진행료총결:(1)형광표기적표면계산;(2)여모사절기술상결합적형광검측;(3)여DNA련치환상결합적형광기술;(4)여기인침묵기술상결합적형광DNA라집문;(5)여DNA자조장입체결구상결합적형광기술;(6)여DNA변구상결합적형광기술.령일방면,개소료궤충근년래발전기래적신형형광기술:(1)형광신호식별방대기술;(2)여자주기술상결합적형광기술;(3)여PH치변화상결합적DNA형광기술;(4)여miRNAs검측상결합적형광기술.재금후적연구중,지유장저량자긴밀결합,재능발휘DNA계산천연적우세.
DNA computing,as a focus of scientific fields,has developed from simple to complex,from theory to application.In this course,the fluorescence,which can be handled easily and has a sensitive respond,plays an important role.This paper pays more attentions to DNA computing and fluorescence.On one hand,major applications of fluorescence in DNA computing are summed up.There are several aspects:(1)surface computing labeled by fluorescence.(2)The fluorescence detecting combined with enzyme cutting.(3)The fluorescence technology used in DNA hybridization and releasing.(4)The fluorescence logical gate based on RNAi.At first,the expression of fluorescence proteins is controlled by RNAi.(5)The fluorescence technology used in DNA self-assembly.(6)The fluorescence technology based on DNA transforming.On the other hand,several novel methods of fluorescence are introduced as following:(1)Signal amplification of fluorescence molecular beacons.(2)The fluorescence technology associated with magnetic beads.(3)The fluorescence technology changing with PH.(4)Detecting miRNAs with fluorescence technology.Only combine these two aspects into whole,most of the advantages of DNA computing can be taken used entirely.