应用与环境生物学报
應用與環境生物學報
응용여배경생물학보
CHINESE JOURNAL OF APPLIED & ENVIRONMENTAL BIOLOGY
2009年
6期
856-860
,共5页
冯瑞章%姚拓%周万海%龙瑞军
馮瑞章%姚拓%週萬海%龍瑞軍
풍서장%요탁%주만해%룡서군
溶磷菌%生存环境%NaCl浓度%碳源%氮源%溶解氧%磷酸盐%溶磷能力
溶燐菌%生存環境%NaCl濃度%碳源%氮源%溶解氧%燐痠鹽%溶燐能力
용린균%생존배경%NaCl농도%탄원%담원%용해양%린산염%용린능력
phosphate solubilizing bacterium%habitats%NaCl concentration%carbon source%nitrogen source%dissolved oxygen%insoluble phosphate%phosphate solubilization capacity
利用液体培养法研究了不同NaCl浓度、碳源、氮源、溶解氧及磷酸盐对分离自小麦(Triticum aestivum)、苜蓿(Medicago sativa)根际的4株优良溶磷细菌(Lx81、Jm92、Dm84、Lx191)溶磷能力的影响.结果表明:(1)4株溶磷菌均为高耐盐菌株,其中Jm92和Din84维持较高溶磷量的NaCl浓度最高阈值分别为4%和8%,Lx81、Lx191在NaCl浓度超过5%时溶磷活性受到抑制.(2)Lx81的最佳碳源是葡萄糖,其次为蔗糖;另外3株菌在葡萄糖和蔗糖为碳源时,均有较高的溶磷能力;4株菌都几乎不能利用淀粉.(3)Lx81和Jm92在以(NH_4)_2SO_4和NH_4 NO_3为氮源时溶磷活性均较高,(NH_4)_2SO_4 是Lx191的最佳氮源,而NH~+-N存在会导致Dm84溶磷活性降低.(4)供试4株菌均不是专性厌氧菌,但不同菌株对氧的需求量不同.(5)4株溶磷菌对Ca_3-P和Ca_8-P有较大的溶解效率,但它们几乎都不能溶解Ca_(10)-P.图3表4参14
利用液體培養法研究瞭不同NaCl濃度、碳源、氮源、溶解氧及燐痠鹽對分離自小麥(Triticum aestivum)、苜蓿(Medicago sativa)根際的4株優良溶燐細菌(Lx81、Jm92、Dm84、Lx191)溶燐能力的影響.結果錶明:(1)4株溶燐菌均為高耐鹽菌株,其中Jm92和Din84維持較高溶燐量的NaCl濃度最高閾值分彆為4%和8%,Lx81、Lx191在NaCl濃度超過5%時溶燐活性受到抑製.(2)Lx81的最佳碳源是葡萄糖,其次為蔗糖;另外3株菌在葡萄糖和蔗糖為碳源時,均有較高的溶燐能力;4株菌都幾乎不能利用澱粉.(3)Lx81和Jm92在以(NH_4)_2SO_4和NH_4 NO_3為氮源時溶燐活性均較高,(NH_4)_2SO_4 是Lx191的最佳氮源,而NH~+-N存在會導緻Dm84溶燐活性降低.(4)供試4株菌均不是專性厭氧菌,但不同菌株對氧的需求量不同.(5)4株溶燐菌對Ca_3-P和Ca_8-P有較大的溶解效率,但它們幾乎都不能溶解Ca_(10)-P.圖3錶4參14
이용액체배양법연구료불동NaCl농도、탄원、담원、용해양급린산염대분리자소맥(Triticum aestivum)、목숙(Medicago sativa)근제적4주우량용린세균(Lx81、Jm92、Dm84、Lx191)용린능력적영향.결과표명:(1)4주용린균균위고내염균주,기중Jm92화Din84유지교고용린량적NaCl농도최고역치분별위4%화8%,Lx81、Lx191재NaCl농도초과5%시용린활성수도억제.(2)Lx81적최가탄원시포도당,기차위자당;령외3주균재포도당화자당위탄원시,균유교고적용린능력;4주균도궤호불능이용정분.(3)Lx81화Jm92재이(NH_4)_2SO_4화NH_4 NO_3위담원시용린활성균교고,(NH_4)_2SO_4 시Lx191적최가담원,이NH~+-N존재회도치Dm84용린활성강저.(4)공시4주균균불시전성염양균,단불동균주대양적수구량불동.(5)4주용린균대Ca_3-P화Ca_8-P유교대적용해효솔,단타문궤호도불능용해Ca_(10)-P.도3표4삼14
The effects of different NaCl concentrations, carbon sources, nitrogen sources, dissolved oxygen and insoluble phosphates on phosphate solubilizing activity of four phosphate solubilizing bacterial (PSB) strains isolated from rhizosphere of wheat (Triticum aestivum) and alfalfa (Medicago sativa) were studied in liquid media. The results showed that the four isolates had high phosphate solubilizing capacity to tolerate salt stress. Strains Jm92 and Dm84 could tolerate NaCl at 4% and 8% concentrations, respectively, while strains Lx81 and Lx191 could tolerate NaCl at 5% concentrations. Lx 81 had the highest capacity to solubilize rock phosphate when supplied with glucose, and it was also able to use saccharose to solubilize rock phosphate. The other three strains had higher phosphate solubilization activities when supplied with glucose and saccharose, but all of them used a little starch to solubilize rockphosphate. Lx81 and Jm92 had stronger capacity to dissolve rock phosphate when supplied with (NH_4)_2SO_4 and NH_4NO_3, Lx191 showed strong ability to solubilize rock phosphate only at (NH_4)_2SO_4 medium. However, Dm84 decreased its activities for dissolving rock phosphate at NH_4~+-N medium. The four PSB strains were not obligate anaerobic bacteria, but different strains differed in requirements for O_2. All of them could readily solubilize Ca_3(PO_4)_2 and Ca_8H_2(PO_4)_6·H_2O phosphate. However, they almost could not solubilize (Ca_(10)(PO_4)· F_2 phosphate. Fig 3, Tab 4, Ref14
