CAJ | 학술논문

目的分析2005-2008年福建乙脑病毒毒株的部分基因组序列,探索乙脑病毒临床分离株的变异和进化.方法临床标本(血清或脑脊液)接种BHK-21细胞,分离乙脑病毒,从培养液上清提取病毒RNA,RT-PCR扩增病毒基因组片段,并进行克隆、测序以及序列分析.结果共分离到5株乙脑病毒.对病毒C/prM区240bp和全长E基因的序列分析表明,近几年的这些临床分离株基因型仍然全部属于G3型.种系发生分析结果显示,新分离株与2002、2003年分离株存在较大同源性.虽然病毒E基因存在少量氨基酸改变,但总体上这些变异不足以影响病毒的致病性.结论近几年福建省的临床乙脑病毒分离株为G3型,新分离株亲缘关系比较接近2002、2003年毒株.
목적 분석2005-2008년복건을뇌병독독주적부분기인조서렬,탐색을뇌병독림상분리주적변이화진화.방법 림상표본(혈청혹뇌척액)접충BHK-21세포,분리을뇌병독,종배양액상청제취병독RNA,RT-PCR확증병독기인조편단,병진행극륭、측서이급서렬분석.결과 공분리도5주을뇌병독.대병독C/prM구240bp화전장E기인적서렬분석표명,근궤년적저사림상분리주기인형잉연전부속우G3형.충계발생분석결과현시,신분리주여2002、2003년분리주존재교대동원성.수연병독E기인존재소량안기산개변,단총체상저사변이불족이영향병독적치병성.결론 근궤년복건성적림상을뇌병독분리주위G3형,신분리주친연관계비교접근2002、2003년독주.
Japanese encephalitis virus (JEV) is an important encephalitis virus in Asia, currently both Genotype I (G1) and Genotype III (G3) strains are circulating in China, while JEV isolates from Fujian belonged to G3. In this report, five clinical JEV strains were isolated from specimens of suspected JE patients in Fujian, 2005-2008. Phylogenetic analysis of partial C/PrM and full-length E genes revealed these five strains also belonged to G3. Meanwhile, homogeneity analysis indicated that 2005-2008 isolates were closely related to strains from several years ago, rather than to those strains from 1950s. Minor variations were identified at several amino acid residues of the envelope protein, however, none of these mutations was associated with pathogenicity of JEV. These data contribute to continuous tracking of the geographic evolution of JEV over a long period.