CAJ | 학술논문

应用十六烷基三甲基溴化铵(CTAB)作为表面活性剂修饰单壁碳纳米管,研究CTAB修饰后的单壁碳纳米管的分散情况和表面电荷情况;观察在场发射扫描电子显微镜和透射电子显微镜下的形貌,同时还研究CTAB修饰后的单壁碳纳米管与小干扰RNA结合的最佳浓度配比,以及CTAB功能修饰后的碳纳米管对培养的人脐静脉内皮细胞的毒性.结果表明:CTAB修饰后的单壁碳纳米管分散良好,CTAB吸附到单根或成束的碳纳米管管壁上,表面带正电荷;与带负电荷的寡核苷酸分子小干扰RNA可以结合,并且CTAB-SWNT与小干扰RNA结合比例达到1:1.5到1:2之间时基本饱和;缺乏CTAB的单壁碳纳米管不能结合小干扰RNA;没有分散的单壁碳纳米管具有更大的细胞毒性,CTAB可以改善SWNTs的分散性,从而减轻单壁碳纳米管的细胞毒性.
응용십륙완기삼갑기추화안(CTAB)작위표면활성제수식단벽탄납미관,연구CTAB수식후적단벽탄납미관적분산정황화표면전하정황;관찰재장발사소묘전자현미경화투사전자현미경하적형모,동시환연구CTAB수식후적단벽탄납미관여소간우RNA결합적최가농도배비,이급CTAB공능수식후적탄납미관대배양적인제정맥내피세포적독성.결과표명:CTAB수식후적단벽탄납미관분산량호,CTAB흡부도단근혹성속적탄납미관관벽상,표면대정전하;여대부전하적과핵감산분자소간우RNA가이결합,병차CTAB-SWNT여소간우RNA결합비례체도1:1.5도1:2지간시기본포화;결핍CTAB적단벽탄납미관불능결합소간우RNA;몰유분산적단벽탄납미관구유경대적세포독성,CTAB가이개선SWNTs적분산성,종이감경단벽탄납미관적세포독성.
Functionalized carbon nanotubes (CNTs) were made for the delivery of genes and drugs and CNT-based biosensors. The basis of CNTs is for binding with biomolecules in biomedical applications. The binding tendency with small interfering RNA oligonucleotides and cytotoxicity of cetyltrimethyl ammonium bromide (CTAB)-coated single-walled carbon nanotubes (SWNTs) were studied. The field emission scanning electron microscopy and transmission electron microscopy results show that a SWNT suspension in CTAB solution was well-dispersed and stable. CTAB is the cross-linker between SWNTs and oligonucleotides. The CTAB-coated SWNTs have less cytotoxicity to human umbilical vein endothelial cells than single SWNTs and the cytotoxicity of CTAB-coated SWNTs depended on the concentration of CTAB-coated SWNTs.