CAJ | 학술논문

目的探讨乌司他丁对大鼠肢体缺血再灌注时肺组织NF-κB表达的影响.方法雄性SD大鼠48只,体重230～260 g,采用随机数字表,将其随机分为3组(n=16):假手术组(S组)、肢体缺血再灌注组(I/R组)和乌司他丁组(U组).I/R组和U组采用夹闭双侧股动脉2 h再开放的方法制备后肢缺血再灌注诱发肺损伤模型.分别于再灌注2、4 h时处死8只大鼠,取肺组织,计算肺组织湿/干重比,采用免疫组化法测定NF-κB表达水平,光镜下观察病理学结果.结果与S组比较,I/R组肺组织湿/干重比升高,肺组织NF-κB表达上调(P＜0.05);与I/R组比较,U组肺组织湿/干重比降低,肺组织NF-κB表达下调(P＜0.05).U组肺组织损伤程度轻于I/R组.结论乌司他丁可下调肺组织NFκB表达,从而减轻大鼠肢体缺血再灌注诱发肺损伤.
목적 탐토오사타정대대서지체결혈재관주시폐조직NF-κB표체적영향.방법 웅성SD대서48지,체중230～260 g,채용수궤수자표,장기수궤분위3조(n=16):가수술조(S조)、지체결혈재관주조(I/R조)화오사타정조(U조).I/R조화U조채용협폐쌍측고동맥2 h재개방적방법 제비후지결혈재관주유발폐손상모형.분별우재관주2、4 h시처사8지대서,취폐조직,계산폐조직습/간중비,채용면역조화법측정NF-κB표체수평,광경하관찰병이학결과.결과 여S조비교,I/R조폐조직습/간중비승고,폐조직NF-κB표체상조(P＜0.05);여I/R조비교,U조폐조직습/간중비강저,폐조직NF-κB표체하조(P＜0.05).U조폐조직손상정도경우I/R조.결론 오사타정가하조폐조직NFκB표체,종이감경대서지체결혈재관주유발폐손상.
Objective To investigate the effects of ulinastatin on the expression of NF-κB in lung tissues during limb ischemia-reperfusion(I/R) in rats.Methods Forty-eight male SD rats weighing 230-260 g were randomly divided into 3 groups (n=16 each):sham operation group (group S);I/R group; ulinastatin group (group U).A rat model of lung injury induced by I/R of hind limbs which was produced by occlusion of the bilateral femoral arteries for 2 h followed by reperfusion was established.The rats were sacrificed at 2 and 4 h of reperfusion(8 rats at each time point) and the lung tissues removed for determination of NF-κB expression (by immuno-histochemistry) and microscopic examination.W/D lung weight ratio was calculated.Results W/D lung weight ratio was significantly increased and NF-κB expression was up-regulated in group I/R compared with group S(P＜ 0.05). W/D lung weight ratio was significantly decreased and NF-κB expression was down-regulated in group U compared with group I/R (P＜0.05). The lung injury induced by I/R was reduced in group U compared with group I/R. Conclusion Ulinastatin can reduce the lung injury induced by limb I/R by down-regulating the expression of NF-κB in rat lung tissues.