国际儿科学杂志
國際兒科學雜誌
국제인과학잡지
INTERNATIONAL JOURNAL OF PEDIATRICS
2010年
1期
3-5,封3
,共4页
热性惊厥%热应激%胆囊收缩素%海马
熱性驚厥%熱應激%膽囊收縮素%海馬
열성량궐%열응격%담낭수축소%해마
Febrile convulsion%Heat stress%Cholecystokinin%Hppocampus
目的 探讨热应激(heat stress,Hs)和热惊厥(febrile convulsions,FC)对大鼠海马和大脑皮层胆囊收缩素蛋白(cholecystokinin,CCK)表达的影响.方法 采用热水浴诱导21日龄大鼠FC模型,应用免疫组织化学技术对HS和FC大鼠CCK在海马和大脑皮层的表达进行分析.结果 对照组中,大脑皮层偶见CCK零星表达.HS组CCK在大脑皮层有较弱表达,主要分布于杏仁周皮质、内嗅皮质和压部后皮质,海马未见表达.而FC组则于大脑皮层、海马见大量CCK强阳性细胞,呈弥漫性分布,无明显核团分布特征.结论 CCK在FC组中大脑皮层和海马各区明显表达,表明CCK参与海马等前脑结构对惊厥活动的中枢调控机制.
目的 探討熱應激(heat stress,Hs)和熱驚厥(febrile convulsions,FC)對大鼠海馬和大腦皮層膽囊收縮素蛋白(cholecystokinin,CCK)錶達的影響.方法 採用熱水浴誘導21日齡大鼠FC模型,應用免疫組織化學技術對HS和FC大鼠CCK在海馬和大腦皮層的錶達進行分析.結果 對照組中,大腦皮層偶見CCK零星錶達.HS組CCK在大腦皮層有較弱錶達,主要分佈于杏仁週皮質、內嗅皮質和壓部後皮質,海馬未見錶達.而FC組則于大腦皮層、海馬見大量CCK彊暘性細胞,呈瀰漫性分佈,無明顯覈糰分佈特徵.結論 CCK在FC組中大腦皮層和海馬各區明顯錶達,錶明CCK參與海馬等前腦結構對驚厥活動的中樞調控機製.
목적 탐토열응격(heat stress,Hs)화열량궐(febrile convulsions,FC)대대서해마화대뇌피층담낭수축소단백(cholecystokinin,CCK)표체적영향.방법 채용열수욕유도21일령대서FC모형,응용면역조직화학기술대HS화FC대서CCK재해마화대뇌피층적표체진행분석.결과 대조조중,대뇌피층우견CCK령성표체.HS조CCK재대뇌피층유교약표체,주요분포우행인주피질、내후피질화압부후피질,해마미견표체.이FC조칙우대뇌피층、해마견대량CCK강양성세포,정미만성분포,무명현핵단분포특정.결론 CCK재FC조중대뇌피층화해마각구명현표체,표명CCK삼여해마등전뇌결구대량궐활동적중추조공궤제.
Objective To analyze the expression of cholecystokinin(CCK) in rat' s hippocampus and cerebral cortex following heat stress and febrile convulsions (FC). Methods Warm water was used to induce FC model. Immunohistochemistry method tested CCK protein. Results Very few or no CCK neurons were seen in the control rats. There was weaker expression of CCK in cerebral cortex of HS group,mainly located in PIR,ENT and RS subfield.No CCK positive ceiis were seen in hippocampus of HS rats. In contrast, abundant expression of CCK protein was found in hippocampus and cerebral cortex of FC group. They were well-distributed, without characteristic subnucleus-specific distribution. Conclusion Abundant induction of CCK in hippocampus and cerebral cortex of FC group suggested that CCK may play a role in the central control of FC.
