中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2009年
5期
343-351
,共9页
计算机%分子%细胞黏附%真皮模板%微纳米印刷%桥墩样结构阵列培养基质%细胞
計算機%分子%細胞黏附%真皮模闆%微納米印刷%橋墩樣結構陣列培養基質%細胞
계산궤%분자%세포점부%진피모판%미납미인쇄%교돈양결구진렬배양기질%세포
Computers%molecular%Cell adhesion%Dermal template%Micronano-printing%Micropier grid culture matrix%cell
目的 从微观角度探讨真皮基质三维结构对Fb生物学行为的影响.方法 借鉴平面几何、三角函数分析真皮组织三维结构,按照真皮组织由黏附成分和非黏附成分组成的原理,通过计算机辅助设计不同参数具有细胞黏着作用的点状结构阵列;运用微图案印刷和分子自组装法,建立4种(8μm×3 μm、间距6 μm,16 μm×3μm、间距6 μm,16 μm×5μm、间距8 μm,20 μm×3 μm、间距2 μm)具有细胞黏附点的桥墩样结构阵列细胞培养基质(MPGCC)培养人Fb,以无MPGCC培养的Fb为对照.利用免疫组织化学、荧光免疫组织化学、噻唑蓝法、羟脯氨酸含量测定法,检测培养Fb中骨架蛋白α-平滑肌肌动蛋白(α-SMA)表达、细胞活力和细胞分泌情况.结果 数学推导结果提示,真皮组织三维结构可用MPGCC进行模拟.用上述4种规格MPGCC培养的Fb其α-SMA表达百分率依次为(49±3)%、(61±3)%、(47±4)%、(51±3)%,与对照组(12±3)%比较,差异有统计学意义(P<0.05);Fb活力依次为0.12±0.03、0.13±0.04、0.14±0.03、0.19±0.03,与对照组0.35±0.04比较显著下降(P<0.05);羟脯氨酸含量依次为(0.95±0.04)、(0.87±0.03)、(0.81±0.03)、(0.77±0.03)μg/mg,与对照组(0.53±0.03)μg/mg比较显著上升(P<0.05).通过调整桥墩角度和桥墩间阵列参数,4组阵列相互对比,α-SMA表达、细胞活力和羟脯氨酸含量差异均有统计学意义(P<0.05).结论 MPGCC可能是真皮模板的基本功能单位或称为真皮模板单元,不同的真皮组织三维环境可产生不同的模板效应和创面愈合结局.
目的 從微觀角度探討真皮基質三維結構對Fb生物學行為的影響.方法 藉鑒平麵幾何、三角函數分析真皮組織三維結構,按照真皮組織由黏附成分和非黏附成分組成的原理,通過計算機輔助設計不同參數具有細胞黏著作用的點狀結構陣列;運用微圖案印刷和分子自組裝法,建立4種(8μm×3 μm、間距6 μm,16 μm×3μm、間距6 μm,16 μm×5μm、間距8 μm,20 μm×3 μm、間距2 μm)具有細胞黏附點的橋墩樣結構陣列細胞培養基質(MPGCC)培養人Fb,以無MPGCC培養的Fb為對照.利用免疫組織化學、熒光免疫組織化學、噻唑藍法、羥脯氨痠含量測定法,檢測培養Fb中骨架蛋白α-平滑肌肌動蛋白(α-SMA)錶達、細胞活力和細胞分泌情況.結果 數學推導結果提示,真皮組織三維結構可用MPGCC進行模擬.用上述4種規格MPGCC培養的Fb其α-SMA錶達百分率依次為(49±3)%、(61±3)%、(47±4)%、(51±3)%,與對照組(12±3)%比較,差異有統計學意義(P<0.05);Fb活力依次為0.12±0.03、0.13±0.04、0.14±0.03、0.19±0.03,與對照組0.35±0.04比較顯著下降(P<0.05);羥脯氨痠含量依次為(0.95±0.04)、(0.87±0.03)、(0.81±0.03)、(0.77±0.03)μg/mg,與對照組(0.53±0.03)μg/mg比較顯著上升(P<0.05).通過調整橋墩角度和橋墩間陣列參數,4組陣列相互對比,α-SMA錶達、細胞活力和羥脯氨痠含量差異均有統計學意義(P<0.05).結論 MPGCC可能是真皮模闆的基本功能單位或稱為真皮模闆單元,不同的真皮組織三維環境可產生不同的模闆效應和創麵愈閤結跼.
목적 종미관각도탐토진피기질삼유결구대Fb생물학행위적영향.방법 차감평면궤하、삼각함수분석진피조직삼유결구,안조진피조직유점부성분화비점부성분조성적원리,통과계산궤보조설계불동삼수구유세포점착작용적점상결구진렬;운용미도안인쇄화분자자조장법,건립4충(8μm×3 μm、간거6 μm,16 μm×3μm、간거6 μm,16 μm×5μm、간거8 μm,20 μm×3 μm、간거2 μm)구유세포점부점적교돈양결구진렬세포배양기질(MPGCC)배양인Fb,이무MPGCC배양적Fb위대조.이용면역조직화학、형광면역조직화학、새서람법、간포안산함량측정법,검측배양Fb중골가단백α-평활기기동단백(α-SMA)표체、세포활력화세포분비정황.결과 수학추도결과제시,진피조직삼유결구가용MPGCC진행모의.용상술4충규격MPGCC배양적Fb기α-SMA표체백분솔의차위(49±3)%、(61±3)%、(47±4)%、(51±3)%,여대조조(12±3)%비교,차이유통계학의의(P<0.05);Fb활력의차위0.12±0.03、0.13±0.04、0.14±0.03、0.19±0.03,여대조조0.35±0.04비교현저하강(P<0.05);간포안산함량의차위(0.95±0.04)、(0.87±0.03)、(0.81±0.03)、(0.77±0.03)μg/mg,여대조조(0.53±0.03)μg/mg비교현저상승(P<0.05).통과조정교돈각도화교돈간진렬삼수,4조진렬상호대비,α-SMA표체、세포활력화간포안산함량차이균유통계학의의(P<0.05).결론 MPGCC가능시진피모판적기본공능단위혹칭위진피모판단원,불동적진피조직삼유배경가산생불동적모판효응화창면유합결국.
Objective To explore the effect of three-dimensional structure of dermal matrix on bio-logical behavior of fibroblasts (Fb) in the microcosmic perspective. Methods The three-dimensional structure of dermal tissue was analyzed by plane geometric and trigonometric function. Microdots structure ar-ray with cell adhesion effect was designed by computer-assisted design software according to the adhesive and non-adhesive components of dermal tissue. Four sizes (8 μm × 3 μm, space 6 μm ;16 μm× 3μm, space 6 μm;16 μm×5 μm, space 8 μm;20 μm × 3 μm, space 2 μm) of micropier grid used for cell culture (MPGCC) with cell-adhesive microdots, built up with micro-pattern printing and molecule self-assembly method were used to culture dermal Fb. Fb cultured with cell culture matrix without micropier grid was set up as control. The expression of skeleton protein (α-SMA) of Fb, cell viability and cell secretion were de-tected with immunohistochemistry, fluorescent immunohistochemistry, MTT test and the hydroxyproline con-tent assay. Results The three-dimensional structure of dermal tissue could be simulated by MPGCC as shown in arithmetic analysis. Compared with those of control group [(12 ± 3)% and (0.53 ± 0.03) μg/mg, (0.35 ±0.04)] , the expression of α-SMA[(49±3)% , (61 ±3)% , (47±4) % , (51±3)%] and the content of hydroxyproline [(0.95±0.04), (0.87±0.03), (0.81±0.03), (0.77±0.03) μg/mg] were increased significantly (P <0.05), the cell viability of Fb (0. 12±0.03, 0. 13±0.04, 0.14± 0.03, 0.19± 0.03) cultured in MPGCC was decreased significantly (P < 0.05). When the parameters of micropier grid were changed, the expression of α-SMA, the cell viability and the content of hydroxyproline of Fb cultured in four sizes of MPGCC were also significantly changed as compared with one another (P < 0.05). Conclusions MPGCC may be the basic functional unit of dermal template, or unit of dermal tem-plate to call. Different three-dimensional circumstances for dermal tissue can result in different template effect and wound healing condition.
