CAJ | 학술논문

目的研究肥胖及2型糖尿病患者皮下和腹内脂肪组织视黄醇结合蛋白4(RBP4)mRNA的表达差异,并探讨影响其表达调控的因素.方法选取2007年1-6月因非代谢性疾病而接受腹部择期手术的正常体重正常糖调节、单纯肥胖、2型糖尿病患者各9例,取皮下和腹内脂肪组织,用RT-PCR法检测RBP4 mRNA的表达,并在体外对正常体重正常糖调节者腹内脂肪组织分别与一定浓度的胰岛素、地塞米松、棕榈酸、肿瘤坏死因子-α、吡咯列酮共培养,用RT-PCR法检测药物对腹内脂肪组织RBP4 mRNA表达的变化.结果肥胖和2型糖尿病患者腹内脂肪RBP4 mRNA的表达均显著高于正常体重正常糖调节者(分别为2.10±1.84和1.54±0.46比0.75±0.28,P＜0.01和P＜0.05),并明显高于相应的皮下脂肪组织;3组人群间皮下脂肪组织RBP4 mRNA的表达差异无统计学意义(1.05±0.15比0.99±0.14比1.13±0.07,P＞0.05);药物胰岛素、地塞米松、吡咯列酮、棕榈酸能显著上调RBP4 mRNA的表达,与对照组相比,分别上升了2.13、0.84、2.04、4.88倍;肿瘤坏死因子-α显著下调RBP4 mRNA的表达,较对照组下降了38%.结论肥胖和2型糖尿病患者腹内脂肪组织RBP4 mRNA的表达显著上升,正常体重正常糖调节者腹内脂肪组织RBP4 mRNA的表达受胰岛素、地塞米松等多种参与糖脂代谢及胰岛素抵抗因素的调控.
목적 연구비반급2형당뇨병환자피하화복내지방조직시황순결합단백4(RBP4)mRNA적표체차이,병탐토영향기표체조공적인소.방법 선취2007년1-6월인비대사성질병이접수복부택기수술적정상체중정상당조절、단순비반、2형당뇨병환자각9례,취피하화복내지방조직,용RT-PCR법검측RBP4 mRNA적표체,병재체외대정상체중정상당조절자복내지방조직분별여일정농도적이도소、지새미송、종려산、종류배사인자-α、필각렬동공배양,용RT-PCR법검측약물대복내지방조직RBP4 mRNA표체적변화.결과 비반화2형당뇨병환자복내지방RBP4 mRNA적표체균현저고우정상체중정상당조절자(분별위2.10±1.84화1.54±0.46비0.75±0.28,P＜0.01화P＜0.05),병명현고우상응적피하지방조직;3조인군간피하지방조직RBP4 mRNA적표체차이무통계학의의(1.05±0.15비0.99±0.14비1.13±0.07,P＞0.05);약물이도소、지새미송、필각렬동、종려산능현저상조RBP4 mRNA적표체,여대조조상비,분별상승료2.13、0.84、2.04、4.88배;종류배사인자-α현저하조RBP4 mRNA적표체,교대조조하강료38%.결론 비반화2형당뇨병환자복내지방조직RBP4 mRNA적표체현저상승,정상체중정상당조절자복내지방조직RBP4 mRNA적표체수이도소、지새미송등다충삼여당지대사급이도소저항인소적조공.
Objective To study the RBP4 mRNA expression between subcutaneous and visceral adipose tissue in obese and type 2 diabetic patients and to investigate the factors that influence RBP4 mRNA expression in Human visceral adipose tissue. Methods 9 individuals with normal weight normal glucose regulation subjects, 9 obesity subjects and 9 type 2 diabetes subjects were enrolled. All of the subjects were prepared to undergone an operation because of nondiabetes disease. Subcutaneous and visceral adipose tissue were taken out as soon as cultured. RT-PCR and Real-time PCR were used to assay the relative expression of RBP4 mRNA. Results RBP4 mRNA level in visceral adipose tissue of obesity group was (2. 10 ± 1.84),and that of type 2 diabetes group was ( 1.54 ± 0. 46 ), both were significantly higher than that in normal weight normal glucose group ( 0. 75 ± 0. 28, P ＜ 0. 01 , P ＜ 0. 05 ). RBP4 mRNA level in subcutaneous adipose tissue of the three groups were ( 1.05 ±0. 15 vs 0. 99 ±0. 14 vs 1.13 ±0. 07), no difference among them(P ＞ 0. 05 ). Insulin, dexamethasone, pioglitazone, free fatty acids can significantly increase RBP4 mRNA expression, compared with the control group, respectively, have an increase of 2. 13 times, 0. 84times, 2. 04 times, 4. 88 times; however, tumor necrosis factor-αt can significantly lower RBP4 mRNA level, compared with the control group decreased by 38%. Conclusion RBP4 mRNA expression in visceral adipose tissue were significantly higher in obesity and type 2 diabetes subjects. In vitro system, RBP4 gene expression in visceral adipose tissue of normal weight normal glucose subjects was regulated by insulin,dexamethasone, pioglitazone, palmitic acid and TNF-α, such factors were also participated in the pathophysiological process of insulin resistance and type 2 diabetes.