CAJ | 학술논문

Paf1复合体己在酵母菌被发现,并阐明了其在转录及RNA加工过程中发挥功能.我们在果蝇基因组数据库中鉴定出paf1的三个同源成分:paf1,CDC73和RTF1.应用纯化的重组蛋白,我们制备了抗果蝇paf1,CDC73和RTF1的抗体.这些抗体不但能够检测出果蝇内源性paf1成分,而且能检测出人类的paf1复合体的亚基.在多线染色体上,这些抗体所检测到的paf1复合体均位于转录的活性位点,并且与磷酸化的RNA聚合酶重合,表明果蝇paf1复合体参与了转录及其相关的分子活动.
Paf1복합체기재효모균피발현,병천명료기재전록급RNA가공과정중발휘공능.아문재과승기인조수거고중감정출paf1적삼개동원성분:paf1,CDC73화RTF1.응용순화적중조단백,아문제비료항과승paf1,CDC73화RTF1적항체.저사항체불단능구검측출과승내원성paf1성분,이차능검측출인류적paf1복합체적아기.재다선염색체상,저사항체소검측도적paf1복합체균위우전록적활성위점,병차여린산화적RNA취합매중합,표명과승paf1복합체삼여료전록급기상관적분자활동.
Paf1 complex was identified in yeast and characterized to function in transcription and its related events.We identified the Drosophila homological components of paf1,CDC73 and RTF1 of paf1 complex.The genes encoding Drosophila paf1,CDC73 and RTF1 were cloned and expressed.With the purified recombinant proteins of truncated components of paf1 complex,antibodies against the Drosophila paf1,CDC73 and RTF1 were generated.These antibodies have been shown to be able to detect the endogenous paf1 subunits as well as their human counterparts in the HeLa extract.On Drosophila polytene chromosomes,these antibodies have beendemonstrated to locate the paf1 complex at actively transcribing sites,which CO-localized with phosphorylated RNA polymerase Ⅱ,indicating that paf1 complex in Drosophila is involved in transcription or the events coupling with transcription.