天津医药
天津醫藥
천진의약
TIANJIN MEDICAL JOURNAL
2009年
7期
592-594,后插4
,共4页
睾酮%精细胞%细胞凋亡%显微镜检查,电子%流式细胞术%原位缺口%末端标记%大鼠,Sprague-Dawley
睪酮%精細胞%細胞凋亡%顯微鏡檢查,電子%流式細胞術%原位缺口%末耑標記%大鼠,Sprague-Dawley
고동%정세포%세포조망%현미경검사,전자%류식세포술%원위결구%말단표기%대서,Sprague-Dawley
testosterone%spermatids%apoptosis%microscopy,electron%flow eytometry%in situ nick-end labeling%rats,Sprague-Dawley
目的:研究睾酮类避孕药丙酸睾丸酮对大鼠生精细胞凋亡的影响及其机制.方法:30只35日龄雄性SD大鼠,随机分为实验组和对照组,每组15只.实验组每天肌内注射内酸睾丸酮40 mg/kg,对照组注射生理盐水.于大鼠出生第50天取血,并处死大鼠切取双侧睾丸.应用原位末端标记检测、流式细胞测定(FCM)、电镜及放射免疫等技术,对大鼠睾丸生精细胞的凋亡进行定性、定位和定量研究.结果:与对照组相比,实验组大鼠睾丸生精细胞凋亡数量增多,主要为初级精母细胞;实验组、对照组大鼠生殖细胞凋亡率分别为(11.3±0.9)%和(3.6±2.3)%,1C峰细胞的百分比分别为(21.8±7.1)%和(33.8±7.5)%,2C峰细胞的百分比分别为(52.6±8.2)%和(37.1±12.5)%,差别均有统计学意义(P＜0.01).实验组和对照组大鼠血清中睾酮水平分别为(3486.8±333.3)ns/L和(846.9±167.5)ng/L,卵泡刺激素水平分别为(2.5±0.8)IU/L和(5.2±1.7)IU/L.差别均有统计学意义(P＜0.01).结论:外源性丙酸睾丸酮可能通过反馈性抑制垂体性腺轴而诱导生精细胞凋亡,发挥其避孕的作用.
目的:研究睪酮類避孕藥丙痠睪汍酮對大鼠生精細胞凋亡的影響及其機製.方法:30隻35日齡雄性SD大鼠,隨機分為實驗組和對照組,每組15隻.實驗組每天肌內註射內痠睪汍酮40 mg/kg,對照組註射生理鹽水.于大鼠齣生第50天取血,併處死大鼠切取雙側睪汍.應用原位末耑標記檢測、流式細胞測定(FCM)、電鏡及放射免疫等技術,對大鼠睪汍生精細胞的凋亡進行定性、定位和定量研究.結果:與對照組相比,實驗組大鼠睪汍生精細胞凋亡數量增多,主要為初級精母細胞;實驗組、對照組大鼠生殖細胞凋亡率分彆為(11.3±0.9)%和(3.6±2.3)%,1C峰細胞的百分比分彆為(21.8±7.1)%和(33.8±7.5)%,2C峰細胞的百分比分彆為(52.6±8.2)%和(37.1±12.5)%,差彆均有統計學意義(P＜0.01).實驗組和對照組大鼠血清中睪酮水平分彆為(3486.8±333.3)ns/L和(846.9±167.5)ng/L,卵泡刺激素水平分彆為(2.5±0.8)IU/L和(5.2±1.7)IU/L.差彆均有統計學意義(P＜0.01).結論:外源性丙痠睪汍酮可能通過反饋性抑製垂體性腺軸而誘導生精細胞凋亡,髮揮其避孕的作用.
목적:연구고동류피잉약병산고환동대대서생정세포조망적영향급기궤제.방법:30지35일령웅성SD대서,수궤분위실험조화대조조,매조15지.실험조매천기내주사내산고환동40 mg/kg,대조조주사생리염수.우대서출생제50천취혈,병처사대서절취쌍측고환.응용원위말단표기검측、류식세포측정(FCM)、전경급방사면역등기술,대대서고환생정세포적조망진행정성、정위화정량연구.결과:여대조조상비,실험조대서고환생정세포조망수량증다,주요위초급정모세포;실험조、대조조대서생식세포조망솔분별위(11.3±0.9)%화(3.6±2.3)%,1C봉세포적백분비분별위(21.8±7.1)%화(33.8±7.5)%,2C봉세포적백분비분별위(52.6±8.2)%화(37.1±12.5)%,차별균유통계학의의(P＜0.01).실험조화대조조대서혈청중고동수평분별위(3486.8±333.3)ns/L화(846.9±167.5)ng/L,란포자격소수평분별위(2.5±0.8)IU/L화(5.2±1.7)IU/L.차별균유통계학의의(P＜0.01).결론:외원성병산고환동가능통과반궤성억제수체성선축이유도생정세포조망,발휘기피잉적작용.
Objective: To investigate the effects of the exogenous testosterone propionate on apoptosis of rat germ cells and the mechanisms thereof. Methods: Thirty 35-day-old male SD rats were randomly divided into experimental group and the control group. The rats in experimental group were injected (i.m.) testosterone propionate and the control group with an equal volume of saline. By using terminal deoxynueleotidy transferase nediated dUTP nick-end labeling (TUNEL), flow cytometry (FCM), radioimmunoassay (RIA) and electron microscopy, the quantity and quality of apoptosis of germ cells were evaluated. Results:(1) Compared with the control, the apoptotic number of rat germ cells was increased in the experimental group, especially the primary spermatocyte. The apoptotie rate was 11.3% detected by FCM in experimental group,while 3.6% in the control group (P ＜ 0.01). (2) The percentages of 1C were 21.8% in experimental group and 33.8% in control group (P ＜ 0.01).The percentages of 2C were 52.6% in experimental group and 37.1% in control group (P ＜ 0.01). (3) The serum levels of testosterone were (3 486.8±333.3) ng/L in experimental group and (846.9±167.5) ng/L in control group (P ＜ 0.01). The serum levels of follicle-stimulating hormone (FSH) were (2.5±0.8) IU/L in experimental group and (5.2±1.7) IU/L in control group (P ＜0.01). Conclusion: The exogenous testosterone propionate might induce apoptosis of germ cells by retroinhibition of the hypothalamie-pituitary-gonadal axis, thus having contraceptive effects.
