南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2009年
7期
1384-1386
,共3页
哮喘%RhoA%尘螨
哮喘%RhoA%塵螨
효천%RhoA%진만
asthma%Ras homolog gene family,member A%dust mites
目的 探讨RhoA在螨性哮喘患者中的表达及其临床意义.方法 采用免疫组织化学S-P法检测螨性哮喘患者肺泡灌洗液中RhoA蛋白的表达情况,并以非尘螨过敏性哮喘患者及正常人群为对照.结果 螨性哮喘、非螨性哮喘患者和正常对照组RhoA表达阳性率依次为66.7%、70.2%和0,差异具有显著性(P<0.01).不同性别螨性哮喘患者RhoA表达差异无统计学意义(P>0.05).在不同年龄螨性哮喘患者中,儿童组RhoA总阳性率(83.3%)虽较高,与其它组相比,差异无统计学意义(P>0.05).Ⅰ级、Ⅱ级、Ⅲ级和Ⅳ级螨性哮喘患者RhoA表达总阳性率依次为33.3%、57.9%、88.2%和100.0%.差异具有统计学意义(P<0.05).结论 螨性哮喘患者RhoA表达水平高于正常人,且随着临床分级的增加,RhoA表达的阳性率有明显的增大趋势.
目的 探討RhoA在螨性哮喘患者中的錶達及其臨床意義.方法 採用免疫組織化學S-P法檢測螨性哮喘患者肺泡灌洗液中RhoA蛋白的錶達情況,併以非塵螨過敏性哮喘患者及正常人群為對照.結果 螨性哮喘、非螨性哮喘患者和正常對照組RhoA錶達暘性率依次為66.7%、70.2%和0,差異具有顯著性(P<0.01).不同性彆螨性哮喘患者RhoA錶達差異無統計學意義(P>0.05).在不同年齡螨性哮喘患者中,兒童組RhoA總暘性率(83.3%)雖較高,與其它組相比,差異無統計學意義(P>0.05).Ⅰ級、Ⅱ級、Ⅲ級和Ⅳ級螨性哮喘患者RhoA錶達總暘性率依次為33.3%、57.9%、88.2%和100.0%.差異具有統計學意義(P<0.05).結論 螨性哮喘患者RhoA錶達水平高于正常人,且隨著臨床分級的增加,RhoA錶達的暘性率有明顯的增大趨勢.
목적 탐토RhoA재만성효천환자중적표체급기림상의의.방법 채용면역조직화학S-P법검측만성효천환자폐포관세액중RhoA단백적표체정황,병이비진만과민성효천환자급정상인군위대조.결과 만성효천、비만성효천환자화정상대조조RhoA표체양성솔의차위66.7%、70.2%화0,차이구유현저성(P<0.01).불동성별만성효천환자RhoA표체차이무통계학의의(P>0.05).재불동년령만성효천환자중,인동조RhoA총양성솔(83.3%)수교고,여기타조상비,차이무통계학의의(P>0.05).Ⅰ급、Ⅱ급、Ⅲ급화Ⅳ급만성효천환자RhoA표체총양성솔의차위33.3%、57.9%、88.2%화100.0%.차이구유통계학의의(P<0.05).결론 만성효천환자RhoA표체수평고우정상인,차수착림상분급적증가,RhoA표체적양성솔유명현적증대추세.
Objective To detect the expression of RhoA in the bronchoalveolar lavage fluid (BLF) from asthma patients allergic to dust mites and study its clinical significance. Methods BLF was collected from 47 mite-allergic asthma patients, 15 asthma patients non-allergic to dust mites and 7 healthy volunteers to detect RhoA expression by immunohistochemistry.Results The total positivity rates of RhoA expression were 66.7% in mite-allergic asthma patients, 70.2% in asthma patients non-allergic to dust mites and 0 in healthy volunteers, showing significant difference between the 3 groups (P<0.01). No statistical difference was observed in RhoA expression levels between male and female asthma patients allergic to dust mites (P>0.05). The total positivity rate was 83.3% in asthma children allergic to dust mites, higher than that in other patients with different ages, but their RhoA expression levels showed no significant differences (P>0.05). The total positive rates were 33.3%, 57.9%, 88.2% and 100.0% in patients with clinical grades Ⅰ,Ⅱ,Ⅲand Ⅳ, showing significant differences (P<0.05).Conclusions The positivity rate of RhoA in the BLF is higher in asthma patients allergic to dust mites than in normal healthy subjects, increases with the clinical grade.
