CAJ | 학술논문

多溴联苯醚(polybrominated diphenyl ethers,PBDEs)是一类具有内分泌干扰和神经毒性的持久性有机污染物,但对其代谢产物的毒性研究还较少.从细胞毒性和氧化应激的角度,探讨2,2',4,4'.四溴联苯醚(2,2',4,4'-tetrabromodiphenyl ether,BDFA7)的羟基代谢物6-羟基一多溴联苯醚47(6-hydroxylated-2,2',4,4'-tetrapolybrominated diphenyl ethers,6-OH-BDE47)对人肺癌细胞系A549的作用.研究结果发现,6-OH-BDFA7对A549细胞具有明显的细胞毒性,随着染毒剂量增加和时间延长,A549细胞存活率逐渐下降.急性染毒实验证实,6-OH-BDE47对A549细胞的氧化应激系统有严重的影响.当细胞培养体系中6-OH-BDE47浓度≥0.5μmol/L时,24h急性暴露能诱导A549细胞的总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力升高1.03倍以上;而6-OH.BDE47浓度≥2.0μmol/L时,还原性谷胱甘肽(reduced the glutathione,GSH)含量减少71.2%.由此可见,6-OH-BDFA7有一定的细胞毒性,能刺激A549细胞发生氧化应激,诱导抗氧化酶升高和内源性还原剂耗竭.
다추련분미(polybrominated diphenyl ethers,PBDEs)시일류구유내분비간우화신경독성적지구성유궤오염물,단대기대사산물적독성연구환교소.종세포독성화양화응격적각도,탐토2,2',4,4'.사추련분미(2,2',4,4'-tetrabromodiphenyl ether,BDFA7)적간기대사물6-간기일다추련분미47(6-hydroxylated-2,2',4,4'-tetrapolybrominated diphenyl ethers,6-OH-BDE47)대인폐암세포계A549적작용.연구결과발현,6-OH-BDFA7대A549세포구유명현적세포독성,수착염독제량증가화시간연장,A549세포존활솔축점하강.급성염독실험증실,6-OH-BDE47대A549세포적양화응격계통유엄중적영향.당세포배양체계중6-OH-BDE47농도≥0.5μmol/L시,24h급성폭로능유도A549세포적총초양화물기화매(total superoxide dismutase,T-SOD)활력승고1.03배이상;이6-OH.BDE47농도≥2.0μmol/L시,환원성곡광감태(reduced the glutathione,GSH)함량감소71.2%.유차가견,6-OH-BDFA7유일정적세포독성,능자격A549세포발생양화응격,유도항양화매승고화내원성환원제모갈.