CAJ | 학술논문

本文提出了一种新型的抗体固定方法,并利用该方法制备了免疫场效应型传感器用于糖化血红蛋白水平的检测.首先,纳米金颗粒由混合自组装膜(SAMs)包裹形成一种功能化的纳米团簇.然后这种纳米团簇以自组装的方式固定在传感器金电极表面.之后,葡萄糖球菌蛋白ACSPA)被固定在纳米团簇表面用来定向固定抗体.利用该方法,纳米团簇可在金电极表面均匀分布.由此形成的多层生物膜可定向固定抗体,与抗体具有稳定的结合力,能较好地屏蔽噪声干扰,且与场效应型传感器兼容,利用该传感器对糖化血红蛋白和血红蛋白浓度检测,灵敏度分别为152.8 μV·ng-1 ·mL和13.5 μV·ng-·mL.实验结果表明,该传感器具有较好的一致性和准确度,未来有望发展成为一种微型的用于糖尿病监控的糖化血红蛋白传感器.
본문제출료일충신형적항체고정방법,병이용해방법제비료면역장효응형전감기용우당화혈홍단백수평적검측.수선,납미금과립유혼합자조장막(SAMs)포과형성일충공능화적납미단족.연후저충납미단족이자조장적방식고정재전감기금전겁표면.지후,포도당구균단백ACSPA)피고정재납미단족표면용래정향고정항체.이용해방법,납미단족가재금전겁표면균균분포.유차형성적다층생물막가정향고정항체,여항체구유은정적결합력,능교호지병폐조성간우,차여장효응형전감기겸용,이용해전감기대당화혈홍단백화혈홍단백농도검측,령민도분별위152.8 μV·ng-1 ·mL화13.5 μV·ng-·mL.실험결과표명,해전감기구유교호적일치성화준학도,미래유망발전성위일충미형적용우당뇨병감공적당화혈홍단백전감기.
An antibody immobilization method was reported to fabricate an immuno-FET sensor for measuring hemoglobin Ale( HbA1 c) level.Firstly,nanogold particles were capped with mixed self-assembled monolayers (SAMs) to form functional nano spheres.Then,the nano spheres were immobilized on the Au electrode of the immuno-FET sensor.After that,staphylococcal protein A (SPA) was immobilized on the nano spheres of electrode to further bond antibodies.In this way,nano spheres were distributed uniformly on Au electrode to form multi bionic film with the high ability to immobilize antibodies effectively and orientedly and to suppress noise interference.This biofilm was exactly suitable for the feature of FET sensor.Sensitivities of 152.8 μV·ng-1·mL for HbAlc and 13.5 μV·ng-1 ·mL for hemoglobin were exhibited by this sensor.Good consistency and accuracy were achieved.This sensor has the potential to develop into a micro HbAle sensor for the monitoring of diabetes.