CAJ | 학술논문

目的：探讨腺病毒介导的胞嘧啶脱氨酶基因对小鼠肝癌的基因治疗效果。方法：分离纯化AdCD，体外实验观察IC50，旁观者效应及凋亡现象。在体内，AdCD瘤内注射并腹腔注射5FC，观察AdCD/5FC系统对肝癌荷瘤小鼠的抗肿瘤效应。结果：当MOI=100时，AdCD感染的MM45T.Li细胞对5FC敏感，IC50＜50μmol/L。当AdCD感染的MM45T.Li细胞占10%时，可有72%细胞死亡，AdCD/5FC系统对MM45T.Li细胞有明显的旁杀伤效应。TUNEL检测及Hoechst 33258荧光染色证实AdCD/5FC系统可以诱导部分MM45T.Li细胞凋亡。荷瘤小鼠经过治疗21d后，各对照组肿瘤体积均数为2303～2943mm3，治疗组AdCD/5FC组肿瘤体积（365±81）mm3，AdCD/5FC系统治疗小鼠肝癌，可以明显减小肿瘤体积（F＝20.33，P＜0.05＝。对照组中位生存期41～43d，治疗组中位生存期49d。结论：AdCD/5FC系统对小鼠肝癌有明显的治疗作用。
목적：탐토선병독개도적포밀정탈안매기인대소서간암적기인치료효과。방법：분리순화AdCD，체외실험관찰IC50，방관자효응급조망현상。재체내，AdCD류내주사병복강주사5FC，관찰AdCD/5FC계통대간암하류소서적항종류효응。 결과：당MOI=100시，AdCD감염적MM45T.Li세포대5FC민감，IC50＜50μmol/L。당AdCD감염적MM45T.Li세포점10%시，가유72%세포사망，AdCD/5FC계통대MM45T.Li세포유명현적방살상효응。TUNEL검측급Hoechst 33258형광염색증실AdCD/5FC계통가이유도부분MM45T.Li세포조망。하류소서경과치료21d후，각대조조종류체적균수위2303～2943mm3，치료조AdCD/5FC조종류체적（365±81）mm3，AdCD/5FC계통치료소서간암，가이명현감소종류체적（F＝20.33，P＜0.05＝。대조조중위생존기41～43d，치료조중위생존기49d。 결론：AdCD/5FC계통대소서간암유명현적치료작용。
Objective: To investigate anti-tumor effects of AdCD/5FC system on mice bearing MM45T.Li liver cancer. Methods: IC50, bystander effect and cell apoptosis were observed in vitro. After treatment of the tumor with AdCD in situ and injection of 5FC peritoneally into the mice, the anti-tumor effects were observed. Results: When MOI=100, AdCD infected MM45T.Li was sensitive to 5FC, and the IC50 was less than 50μmol/L. When 10% MM45T.Li cells were infected with AdCD, 72% cells died after 5FC treatment, which showed significant bystander effect. Through TUNEL test and Hoechst 33258 staining, it was demonstrated that there was cell apoptosis after AdCD/5FC treatment of the MM45T.Li cell 21d after treatment of the mice bearing tumor. The tumor volume was reduced significantly (F=20.33, P<0.05), and the median survival time was prolonged from 41~43d to 49d. Conclusion: AdCD/5FC system shows significant anti-tumor effects.