CAJ | 학술논문

目的观察热及酸刺激豚鼠食管对气道的影响,探讨瞬时受体电位香草酸亚型1(TRPV1)及神经肽在发生机制中的作用.方法构建豚鼠食管灌流模型.进行持续(20 min)热生理盐水(45℃)和盐酸(0.1 mol/L,37℃)灌流刺激食管,通过伊文思蓝染色法检测其气管、支气管及细支气管的血浆渗出,观察支气管肺泡灌洗液中炎症细胞总数及分类,并观察TRPVl特异性受体拮抗剂CPZ和神经内肽酶抑制剂磷阿米酮对刺激效应的影响.结果与对照组相比(P值均<0.05),热刺激和酸刺激豚鼠食管均可增加其气管、支气管和细支气管血浆渗出;CPZ能抑制热和盐酸刺激引起的气管、支气管血浆渗出增多(P值均<0.05);磷阿米酮能明显增强盐酸和热刺激的效果(P值均<0.05).结论热、酸刺激豚鼠食管会引起气道血浆渗出增多,TRPV1及神经肽可能参与其发生机制.
목적 관찰열급산자격돈서식관대기도적영향,탐토순시수체전위향초산아형1(TRPV1)급신경태재발생궤제중적작용.방법 구건돈서식관관류모형.진행지속(20 min)열생리염수(45℃)화염산(0.1 mol/L,37℃)관류자격식관,통과이문사람염색법검측기기관、지기관급세지기관적혈장삼출,관찰지기관폐포관세액중염증세포총수급분류,병관찰TRPVl특이성수체길항제CPZ화신경내태매억제제린아미동대자격효응적영향.결과 여대조조상비(P치균<0.05),열자격화산자격돈서식관균가증가기기관、지기관화세지기관혈장삼출;CPZ능억제열화염산자격인기적기관、지기관혈장삼출증다(P치균<0.05);린아미동능명현증강염산화열자격적효과(P치균<0.05).결론 열、산자격돈서식관회인기기도혈장삼출증다,TRPV1급신경태가능삼여기발생궤제.
Objective To observe the effect on airway of hot and acid factor stimulated in guinea pigs esophagus,as well as to investigate the mechanism of transient receptor potential vanilloid 1(TRPVl) and neuropeptide in this process.Methods The esophagus of guinea pigs were perfused with hot saline(45℃)and hydrochloric acid(0.1 mol/L,37℃)pretreatment or without TRPV1 antagonist capsazepine and inhibitor of neutral endopeptidase phosphoramidone.The microvascular leakage of trachea,main bronchi and bronchiole were measured by evens blue dye and the total number and percentage of cells in the bronchoalveolar lavage fluid were calculated by HE dye.Results Stimulation by hot saline and 0.1 mol/L hydrochloric acid of the esophagus significantly increased the microvascular leakage of trachea and main bronchi(all P<0.05),microvascular leakage of bronchiole also increased in hot saline group.Phosphoramidone could significantly potentiated plasma extravasation in the trachea,main bronchi and bronchiole(P<0.05); capsazepine inhibited the plasma extravasation of trachea and bronchial induced by hot saline and acid.The total number and percentage of cells in the bronchoalveolar lavage fluid in different groups showed no significant difference.Conclusions Hot saline and hydrochloric acid stimulation intra esophageal can induce microvasclur leakage of airway.TRPV1 and neuropeptide may play an important role in it.