CAJ | 학술논문

目的探讨ARHI蛋白和环氧化酶-2(COX-2)蛋白在子宫颈腺癌发生中的作用及其相互关系.方法采用免疫组织化学法检测40例子宫颈腺癌及30例慢性子宫颈炎组织中ARHI蛋白及COX-2蛋白的表达情况.结果 ARHI在40例子宫颈腺癌组织阳性率为50.0％ (20/40),低于慢性子宫颈炎患者子宫颈组织阳性率90.0％ (27/30),差异有统计学意义(x2=12.43,P＜0.05)；COX-2在40例子宫颈腺癌组织阳性率82.5％(33/40),高于慢性子宫颈炎患者子宫颈组织阳性率0(0/30),差异有统计学意义(x2 =46.16,P＜0.05)；ARHI蛋白阳性表达率与子宫颈腺癌临床分期(x2=3.96,P＜0.05)及组织分化程度高低有关(x2 =7.04,P＜0.05).COX-2蛋白与子宫颈腺癌组织分化程度有关(x2=7.04,P ＜0.05),与临床分期无关(P＞0.05)；ARHI蛋白和COX-2蛋白与淋巴结有无转移均无关(P均＞0.05)；ARHI蛋白和COX-2蛋白表达有关联(r=0.31,P＜0.05).结论子宫颈腺癌的发生与ARHI基因失活和COX-2蛋白表达异常有关,两者可能协同作用参与子宫颈腺癌的发生.
목적 탐토ARHI단백화배양화매-2(COX-2)단백재자궁경선암발생중적작용급기상호관계.방법 채용면역조직화학법검측40례자궁경선암급30례만성자궁경염조직중ARHI단백급COX-2단백적표체정황.결과 ARHI재40례자궁경선암조직양성솔위50.0％ (20/40),저우만성자궁경염환자자궁경조직양성솔90.0％ (27/30),차이유통계학의의(x2=12.43,P＜0.05)；COX-2재40례자궁경선암조직양성솔82.5％(33/40),고우만성자궁경염환자자궁경조직양성솔0(0/30),차이유통계학의의(x2 =46.16,P＜0.05)；ARHI단백양성표체솔여자궁경선암림상분기(x2=3.96,P＜0.05)급조직분화정도고저유관(x2 =7.04,P＜0.05).COX-2단백여자궁경선암조직분화정도유관(x2=7.04,P ＜0.05),여림상분기무관(P＞0.05)；ARHI단백화COX-2단백여림파결유무전이균무관(P균＞0.05)；ARHI단백화COX-2단백표체유관련(r=0.31,P＜0.05).결론 자궁경선암적발생여ARHI기인실활화COX-2단백표체이상유관,량자가능협동작용삼여자궁경선암적발생.
Objective To study the role of ARHI protein and COX-2 protein and to explore their correlation in pathogenesis of adenocarcinoma of the uterine cervix.Methods The expression of ARHI and COX-2 protein were detected both in 40 cases of adenocarcinoma of the uterine cervix and in 30 cases of normal cervix by immunohistochemistry.Results The positive rate of the expression of ARHI protein in adenocarcinoma of the uterine cervix was 50.0％ (20/40),which lower than that in normol cervix controls 90.0％ (27/30),the difference was significant ( x2 =12.43,P ＜ 0.05 ) ; The positive rate of the expression of COX-2 protein in adenocarcinoma of the uterine cervix was 82.5 ％ (33/40),which higher than that in normol cervix controls 0 (0/30),the difference was significant (x2 =46.16,P ＜ 0.05 ) ;The positive expression rate of ARHI was negatively correlated with the clinical stage of cervical adenocarcinoma ( x2 =3.96,P ＜ 0.05 ) disease and pathological grade (x2 =7.04,P ＜ 0.05 ).COX-2 protein was positively correlated with pathological grade (x2 =7.04,P ＜0.05 ),but had no correlation with the clinical stage of cervical adenocarcinomacervical adenocarcinoma stage of diseas(P ＞ 0.05) ;The expression of ARHI protein and COX-2 protein wasn't correlated with lymph nodeatic meatastasis(P ＞ 0.05);The expression of ARHI protein had correlation with the expression of COX-2 protein (r =0.31,P ＜ 0.05 ).Conclusion Inactivation of ARHI gene and the abnormal expression of COX-2 protein are involved in the origin of cervical adenocarcinoma,their synergistic reaction may play an important role in tumor origin of cervical adenocarcinoma.