CAJ | 학술논문

目的探讨Ghrelin对TNF-α诱导的HepG2细胞PAI-1分泌的影响及p38MAPK的作用.方法 HepG2细胞培养,加入TNF-α,ELISA法测定上清PAI-1的含量;免疫印迹法检测磷酸化p38的表达.Ghrelin预处理1 h,检测TNF-α变化.结果 TNF-α浓度依赖地增高PAI-1分泌;Ghrelin组PAI-1减少;TNF-α组p-p38增加,Ghrelin组较TNF-α组减少.结论 TNF-α可能通过p-p38介导HepG2 PAI-1的表达;Ghrelin可能通过抑制p-38通路抑制PAI-1的表达.
목적 탐토Ghrelin대TNF-α유도적HepG2세포PAI-1분비적영향급p38MAPK적작용.방법 HepG2세포배양,가입TNF-α,ELISA법측정상청PAI-1적함량;면역인적법검측린산화p38적표체.Ghrelin예처리1 h,검측TNF-α변화.결과 TNF-α농도의뢰지증고PAI-1분비;Ghrelin조PAI-1감소;TNF-α조p-p38증가,Ghrelin조교TNF-α조감소.결론 TNF-α가능통과p-p38개도HepG2 PAI-1적표체;Ghrelin가능통과억제p-38통로억제PAI-1적표체.
Objective To investigate the effect of ghrelin on PAI-1 secretion in HepG2 cells induced by TNF-αand the effect of p-38 MAPK.Methods HepG2 cells were cultured.The concentration of TNF-α used to treat the HepG2 cells wag selected.The effect of ghrelin on PAI-1 secretion induced by TNF-α was detected by ELISA,the p-38 MAPK expression was investigated by Western blot.Results The concentration of PAI-1 was increased when cells were exposed to different concentration of TNF-α.The p-p38 MAPK expression was increased when the cells were exposed to TNF-α,ghrelin could inhibit the increase of PAI-1 secretioN induced by TNF-α.The expression of p-p38 MAPK was decreased when the cells were pretreated with ghrelin.Conclusion PAI-1 secretion were increased after TNF-α in-creasing.Ghrelin could inhibit PAI-1 secretion via p38 MAPK.