CAJ | 학술논문

目的研究5-羟色胺转运体基因启动子区(5-HTTLPR)、多巴胺D4受体(DRD4)基因上游调控区的-521 C/T多态是否与驾驶员事故倾向性及其A型行为和个性特征存在关联.方法采用病例对照研究,选取事故倾向性驾驶员和正常对照驾驶员各46例.用A型行为量表和EPQ量表进行A型行为及心理评定.采用限制性片段长度多态性分析(PCR- RFLP)技术对5-HTTLPR、DRD4基因-521 C/T多态进行基因型和等位基因频率检测.结果病例组A型行为问卷中时间紧迫感(TH)及TH+无端敌意(CH)分高于对照组,差异有统计学意义(P＜0.05),EPQ问卷中精神质(P)、神经质(N)分量表得分明显高于对照组,真实性(L)分量表分低于对照组,差异均有统计学意义( P＜0.05,P＜0.01).病例组与对照组间5-HTTLPR基因的基因型及等位基因频率分布的差异有统计学意义(P＜0.05),DRD4基因的基因型及等位基因频率分布的差异无统计学意义(P＞0.05).事故倾向性驾驶员5-HTTLPR基因中S(S)基因型的P分量表分高于S/L+L/L基因型,差异有统计学意义(P＜0.05);DRD4基因中TT基因型的E分量表分高于T/C+C/C基因型,差异有统计学意义(P＜0.05).结论 5-HTTLPR基因与驾驶员事故倾向性有关联,而DRD4基因可能与其无关联；这2个基因与事故倾向性驾驶员的A型行为及个性特征有关联；但对这些行为及个性,5-HTTLPR与DRD4基因可能不存在相互协同作用.
목적 연구5-간색알전운체기인계동자구(5-HTTLPR)、다파알D4수체(DRD4)기인상유조공구적-521 C/T다태시부여가사원사고경향성급기A형행위화개성특정존재관련.방법 채용병례대조연구,선취사고경향성가사원화정상대조가사원각46례.용A형행위량표화EPQ량표진행A형행위급심리평정.채용한제성편단장도다태성분석(PCR- RFLP)기술대5-HTTLPR、DRD4기인-521 C/T다태진행기인형화등위기인빈솔검측.결과 병례조A형행위문권중시간긴박감(TH)급TH+무단활의(CH)분고우대조조,차이유통계학의의(P＜0.05),EPQ문권중정신질(P)、신경질(N)분량표득분명현고우대조조,진실성(L)분량표분저우대조조,차이균유통계학의의( P＜0.05,P＜0.01).병례조여대조조간5-HTTLPR기인적기인형급등위기인빈솔분포적차이유통계학의의(P＜0.05),DRD4기인적기인형급등위기인빈솔분포적차이무통계학의의(P＞0.05).사고경향성가사원5-HTTLPR기인중S(S)기인형적P분량표분고우S/L+L/L기인형,차이유통계학의의(P＜0.05);DRD4기인중TT기인형적E분량표분고우T/C+C/C기인형,차이유통계학의의(P＜0.05).결론 5-HTTLPR기인여가사원사고경향성유관련,이DRD4기인가능여기무관련；저2개기인여사고경향성가사원적A형행위급개성특정유관련；단대저사행위급개성,5-HTTLPR여DRD4기인가능불존재상호협동작용.
Objective To investigate the relationship between 5-HTTLPR and/or DRD4 gene polymorphisms and the accident tendentiousness of drivers.Methods A case-control study,including 42 patients and 46 controls,were performed using type-A behavior questionnaire and EPQ scale.5-HTTLPR and DRD4gene -521 C/T were detected by the PCR-RFLP technique.Results The scores of type-A behavior questionnaires,such as TH and TH+CH in exposure group were significantly higher than those in control group (P＜0.05).P and N scores of EPQ questionnaires in exposure group were significantly higher than those in control group,and L score in exposure group was significantly lower than that in control group (P＜0.05 or P＜0.01 ).There were significant differences in the frequencies of the genotypes and alleles of 5-HTTLPR gene between the eases and the controls (P＜0.05),but there were no significant differences in the frequencies of the genotypes and alleles of DRD4 gene between the two groups (P＞0.05).In the drivers with the accident tendentiousness,P scores in the cases with homozygous genotypes of the S/S in 5-HTTLPR gene were significantly higher than those in the caseswith the genotypes of S/L and L/L in 5-HTTLPR gene (P＞0.05).E scores in subjects with homozygous genotypes of the T/T in DRD4 gene were significantly higher than those in subjects with genotypes of the T/C+C/C in DRD4 gene (P＞0.05).Conclusion The driver accident tendentiousness may be associated with 5-HTTLPR gene,but not associated with DRD4 gene.The two genes are associated with the type-A behavior and personality characteristics of drivers with accident tendentiousness.However,5-HTTLPR and DRD4 gene may not have synergism in these behaviors and personality.