CAJ | 학술논문

目的观察正常大脑、脑梗死对侧及梗死侧大脑脑组织上清液对脂肪来源干细胞(ADSCs)向神经元样细胞分化的影响.方法取SD大鼠腹膜后脂肪组织进行ADSCs分离培养;用正常脑组织上清液、脑组织梗死侧及对侧脑组织上清液对ADSCs进行诱导,免疫荧光法检测神经细胞标志物的表达,荧光显微镜下观察细胞阳性率.结果 (1)脑梗死侧组诱导组神经元特异性烯醇酶(NSE)、微管相关蛋白-2(MAP-2)、胶质纤维酸性蛋白(GFAP)表达率均高于其他组(P＜0.05).(2)脑梗死对侧组及正常对照组诱导组NSE、MAP-2、GFAP表达率均高于未干预组(P＜0.05).结论脑梗死侧及脑梗死对侧组织上清液均能诱导大鼠ADSCs分化为神经元样细胞,表达神经细胞标志物,但前者作用更为明显.
목적 관찰정상대뇌、뇌경사대측급경사측대뇌뇌조직상청액대지방래원간세포(ADSCs)향신경원양세포분화적영향.방법 취SD대서복막후지방조직진행ADSCs분리배양;용정상뇌조직상청액、뇌조직경사측급대측뇌조직상청액대ADSCs진행유도,면역형광법검측신경세포표지물적표체,형광현미경하관찰세포양성솔.결과 (1)뇌경사측조유도조신경원특이성희순매(NSE)、미관상관단백-2(MAP-2)、효질섬유산성단백(GFAP)표체솔균고우기타조(P＜0.05).(2)뇌경사대측조급정상대조조유도조NSE、MAP-2、GFAP표체솔균고우미간예조(P＜0.05).결론 뇌경사측급뇌경사대측조직상청액균능유도대서ADSCs분화위신경원양세포,표체신경세포표지물,단전자작용경위명현.
Objective To observe the effect of supernatant liquid from brain tissue on the differentiation of adipose-derived stem cells (ADSCs) into neural cells in normal brain tissues, the homogenate of infarcted cerebral hemisphere and the opposite side in the rats. Methods The ADSCs were obtained from rat retroperitoneal adipose tissue. The normal brain tissues, the homogenate of the infarcted cerebral hemisphere and thc opposite side got from middle cerebral artery occlusion in rats were used to induce ADSCs. Immunocytochemistry or immunofluorescence were used to identify the cell types at the 3rd day. Positive expression rate was counted by fluorescence microscope. Results (1)The neuron-specific enolase (NSE) positive cells, microtubule-associated protein 2 (MAP-2) positive cells and glial fibrillary acidic protein (GFAP) positive cells were much more in the homogenate of the infracted cerebral hemisphere than in others (P＜0.05). (2)The NSE positive cells, MAP-2 positive cells and GFAP positive cells were much more in the homogenate of the normal brain tissues and the opposite side than normal level ( P ＜ 0. 05 ) . Conclusions The homogenate of the infracted cerebral hemisphere and the opposite side can induce adipose-derived stem cells into neural-like cells and express neural cells markers in rats.