中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2008年
11期
1106-1109
,共4页
脑缺血再灌注%血脑屏障%尼莫地平
腦缺血再灌註%血腦屏障%尼莫地平
뇌결혈재관주%혈뇌병장%니막지평
Cerebral ischemia-reperfusion%Blood-brain barrier%Nimodipine
目的 研究钙离子拮抗剂对大鼠脑缺血再灌注后血脑屏障(BBB)通透性和脑梗死灶体积的影响. 方法 插线法制作大鼠脑缺血再灌注模型.缺血2 h后再灌注.将150只大鼠按随机数字表法分尼莫地平组和对照组,每组分再灌注6h、12h、24 h、48h、72 h五个时间段,再灌注后尼莫地平组和对照组立即分别腹腔注射尼莫地平和生理盐水2 mg/kg.每12小时注射一次,用甲酰胺荧光法及透射电镜观察不同时段BBB通透性破坏的情况,TTC染色后计算梗死灶体积百分比.结果 大鼠脑缺血再灌注后BBB通透性和梗死灶体积百分比随时间延长逐渐增加.且BBB通透性的增加呈现两个高峰,第一个高峰在再灌注后12 h,第二个高峰在再灌注后48 h.尼莫地平组BBB通透性及脑梗死灶体积百分比的增加均较对照组明显,差异有统计学意义(P<0.05). 结论 脑缺血再灌注增加BBB的通透性和脑梗死灶体积百分比.再灌注后给予尼莫地平可加重这些病理变化.
目的 研究鈣離子拮抗劑對大鼠腦缺血再灌註後血腦屏障(BBB)通透性和腦梗死竈體積的影響. 方法 插線法製作大鼠腦缺血再灌註模型.缺血2 h後再灌註.將150隻大鼠按隨機數字錶法分尼莫地平組和對照組,每組分再灌註6h、12h、24 h、48h、72 h五箇時間段,再灌註後尼莫地平組和對照組立即分彆腹腔註射尼莫地平和生理鹽水2 mg/kg.每12小時註射一次,用甲酰胺熒光法及透射電鏡觀察不同時段BBB通透性破壞的情況,TTC染色後計算梗死竈體積百分比.結果 大鼠腦缺血再灌註後BBB通透性和梗死竈體積百分比隨時間延長逐漸增加.且BBB通透性的增加呈現兩箇高峰,第一箇高峰在再灌註後12 h,第二箇高峰在再灌註後48 h.尼莫地平組BBB通透性及腦梗死竈體積百分比的增加均較對照組明顯,差異有統計學意義(P<0.05). 結論 腦缺血再灌註增加BBB的通透性和腦梗死竈體積百分比.再灌註後給予尼莫地平可加重這些病理變化.
목적 연구개리자길항제대대서뇌결혈재관주후혈뇌병장(BBB)통투성화뇌경사조체적적영향. 방법 삽선법제작대서뇌결혈재관주모형.결혈2 h후재관주.장150지대서안수궤수자표법분니막지평조화대조조,매조분재관주6h、12h、24 h、48h、72 h오개시간단,재관주후니막지평조화대조조립즉분별복강주사니막지평화생리염수2 mg/kg.매12소시주사일차,용갑선알형광법급투사전경관찰불동시단BBB통투성파배적정황,TTC염색후계산경사조체적백분비.결과 대서뇌결혈재관주후BBB통투성화경사조체적백분비수시간연장축점증가.차BBB통투성적증가정현량개고봉,제일개고봉재재관주후12 h,제이개고봉재재관주후48 h.니막지평조BBB통투성급뇌경사조체적백분비적증가균교대조조명현,차이유통계학의의(P<0.05). 결론 뇌결혈재관주증가BBB적통투성화뇌경사조체적백분비.재관주후급여니막지평가가중저사병리변화.
Objective To study the effect of nimodipine on the blood-brain barrier (BBB) permeability and cerebral infarct volume in mrs after cerebral ischemia and reperfusion. Methods Cerebral ischemia-reperfusion injury was induced by a 2-hour suture occlusion of the unilateral middle cerebral artery followed by reperfusion in 150 rats which were randomized into nimodipine and normal saline groups. Immediately after suture withdrawal to allow reperfusion, the two groups of rats were subjected to intraperitoneal injections of 2 mg/kg nimodipine (nimodipine group) or normal saline (saline group) at a 12-hour interval. At the time points of 6, 12, 24, 48 and 72 h after the reperfusion, the BBB permeability of the rats was evaluated with fluorophotometry and transmission electron microscopy, and the infect volume was estimated using TTC staining. Results The BBB permeability and the percentage of cerebral infarct volume increased gradually with prolonged reperfusion, and the BBB permeability presented with two peak increment occurring at 12 and 48 h of reperfusion, respectively. Nimodipine injection significantly increased the BBB permeability and the infarct volume in comparison with those in the saline group (P<0.05). Conclusion Cerebral ischemia and reperfusion destroys the integrity of the BBB and aggravates the ischemic cerebral infarction in rats, and the use of nimodipine after reperfusion further worsens these pathologies.
