中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
3期
435-437
,共3页
侯敬申%赵莉%覃媛%张曼%黄劭
侯敬申%趙莉%覃媛%張曼%黃劭
후경신%조리%담원%장만%황소
丁酸钠%肝癌%吲哚胺2,3-双加氧酶
丁痠鈉%肝癌%吲哚胺2,3-雙加氧酶
정산납%간암%신타알2,3-쌍가양매
Sodium butyrate%Hepatocellular carcinoma%Indoleamine 2,3-dioxygenase
目的 观察丁酸钠(NaB)对干扰素-γ(IFN-γ)诱导的人肝癌细胞HepG2内吲哚胺2,3-双加氧酶(IDO)表达的影响.方法 利用低浓度(10、20、30、40 mmol/L)的NaB作用于人肝癌细胞HepG2 24 h,通过噻唑蓝(MTT)比色法检测细胞的增殖；利用蛋白印迹法及细胞免疫化学法检测NaB对HepG2细胞内IDO表达的影响;蛋白印迹法检测NaB对IDO基因表达的关键性干扰素应答因子1 (IRF-1)及信号转导和转录激活因子1(STAT1)的影响.结果 低浓度NaB对人肝癌细胞HepG2的增殖具有1％～20％的抑制作用；3 mmol/L的NaB能完全抑制IFN-γ诱导的IDO的表达；这种抑制作用通过抑制STAT-1701位的酪氨酸磷酸化实现.结论 NaB通过抑制STAT1的磷酸化而抑制肝癌细胞HepG2中IDO的表达.
目的 觀察丁痠鈉(NaB)對榦擾素-γ(IFN-γ)誘導的人肝癌細胞HepG2內吲哚胺2,3-雙加氧酶(IDO)錶達的影響.方法 利用低濃度(10、20、30、40 mmol/L)的NaB作用于人肝癌細胞HepG2 24 h,通過噻唑藍(MTT)比色法檢測細胞的增殖；利用蛋白印跡法及細胞免疫化學法檢測NaB對HepG2細胞內IDO錶達的影響;蛋白印跡法檢測NaB對IDO基因錶達的關鍵性榦擾素應答因子1 (IRF-1)及信號轉導和轉錄激活因子1(STAT1)的影響.結果 低濃度NaB對人肝癌細胞HepG2的增殖具有1％～20％的抑製作用；3 mmol/L的NaB能完全抑製IFN-γ誘導的IDO的錶達；這種抑製作用通過抑製STAT-1701位的酪氨痠燐痠化實現.結論 NaB通過抑製STAT1的燐痠化而抑製肝癌細胞HepG2中IDO的錶達.
목적 관찰정산납(NaB)대간우소-γ(IFN-γ)유도적인간암세포HepG2내신타알2,3-쌍가양매(IDO)표체적영향.방법 이용저농도(10、20、30、40 mmol/L)적NaB작용우인간암세포HepG2 24 h,통과새서람(MTT)비색법검측세포적증식；이용단백인적법급세포면역화학법검측NaB대HepG2세포내IDO표체적영향;단백인적법검측NaB대IDO기인표체적관건성간우소응답인자1 (IRF-1)급신호전도화전록격활인자1(STAT1)적영향.결과 저농도NaB대인간암세포HepG2적증식구유1％～20％적억제작용；3 mmol/L적NaB능완전억제IFN-γ유도적IDO적표체；저충억제작용통과억제STAT-1701위적락안산린산화실현.결론 NaB통과억제STAT1적린산화이억제간암세포HepG2중IDO적표체.
Objective To investigate the effect of sodium butyrate (NaB) on the expression of indoleamine 2,3-dioxygenase (IDO) induced by interferon (IFN)-γ in HepG2 cells.Methods HepG2 cells were treated with low concentration of NaB for 24 h,such as 10,20,30 and 40 mmol/L,and the proliferation of the cells was detected by methyl thiazolyl tetrazolium (MTT) assay.The effects of NaB on IDO expression induced by IFN-γ in HepG2 cells were demonstrated by Western blotting and immunocytochemistry.The transcription of interferon responsive factor-1 ( IRF-1 ) and signal transducers and activators of transcription 1 (STAT1),which were key transcription factor regulating IDO expression,were analyzed by Western blotting.Results Low concentration of NaB inhibited the proliferation of HepG2 cells,varying from 1％ to 20％.And the expression of IDO in HepG2 cells was completely inhibited by 3 mmol/L NaB through inhibition of the phosphorylation of STAT1 ( tyrosine at 701 site).Conclusion NaB could inhibit the expression of IDO in HepG2 cells through inhibiting STAT1 phosphorylation.
