CAJ | 학술논문

以鱼油下脚料为原料,以固定化Geotrichum sp.脂肪酶为催化剂,选择性水解粗鱼油,使目标脂肪酸EPA和DHA富集于甘油骨架上,显著提高了甘油酯中EPA和DHA的质量分数.通过单因素试验和响应面分析得出最佳水解条件:2 g粗鱼油,2.5 g水,221.3 U脂肪酶,30 ℃,反应11.7 h.最佳条件下的水解度为42.1%.EPA和DHA质量分数分别为7.8%和41.1%.经过二次水解后,水解度提高到45.9%,EPA和DHA质量分数分别提高到8.5%和42%.与初始鱼油相比,分别提高了2倍和2.2倍.
이어유하각료위원료,이고정화Geotrichum sp.지방매위최화제,선택성수해조어유,사목표지방산EPA화DHA부집우감유골가상,현저제고료감유지중EPA화DHA적질량분수.통과단인소시험화향응면분석득출최가수해조건:2 g조어유,2.5 g수,221.3 U지방매,30 ℃,반응11.7 h.최가조건하적수해도위42.1%.EPA화DHA질량분수분별위7.8%화41.1%.경과이차수해후,수해도제고도45.9%,EPA화DHA질량분수분별제고도8.5%화42%.여초시어유상비,분별제고료2배화2.2배.
Selective hydrolysis of waste fish oil in attempt to enrichment of EPA and DHA glycerides by immobilized Geotrichum sp. lipase was investigated. Through single factor design and response surface analysis, the optimal condition was determined as follows: 2 g crude fish oil, 2.5 g water, 221.3 U lipase, and hydrolysis time 11.7 h at 30 ℃. Under the optimal condition, 42.1% of hydrolysis degree with 7.8% of EPA and 41.1% of DHA was achieved. Moreover, the hydrolysis degree further increased to 45.9%, and the EPA and DHA contents in the resulted glycerides increased to 8.5% and 42% by the second enzymatic hydrolysis reaction, respectively. Compared with original fish oil with 4.2% of EPA and 18.9% of DHA, the contents of EPA and DHA in the resulted glycerides increased to 2-fold and 2.2-fold, respectively.