CAJ | 학술논문

实验研究了太平洋鲑鱼(Oncorhynchus spp.)经8-32d饥饿后对其生长、体组成与血浆生化指标变化的影响.90尾初始重约为217 g的太平洋鲑鱼放养于0.25 m~3的水族箱中0-32d,水温为(17.0±2.9)℃.实验分5组,分别为对照组(饥饿0d)、实验1组(饥饿8d)、实验2组(饥饿16d)、实验3组(饥饿24d)、实验4组(饥饿32d).每组3个平行,每箱6尾鱼.结果表明:饥饿期间,太平洋鲑鱼存活率均为100%,相对体重损失率与饥饿时间直线回归方程为y=0.0086x(R~2=0.9177),呈显著的正相关(P<0.05);肠系膜脂肪是太平洋鲑鱼最主要的能最来源,与饥饿时间呈显著的负相关(P<0.05),其直线回归方程y=-0.0719±4.11(R~2=0.9732);饥饿初期太平洋鲑鱼主要消耗肝脏糖原和脂肪、部分肠系膜脂肪和少许肌肉中脂肪维持生命活动,能耗较低:饥饿后期主要以消耗肠系膜脂肪、部分肌肉脂肪和少量蛋白质维持生命活动,能耗较高;血浆中脂肪分解酶和白蛋白无显著变化(P<0.05),胆同醇、甘油三酯和高低密度脂蛋白有显著波动(P<0.05),表明脂肪代谢活跃,免疫功能未受明显影响.研究表明太平洋鲑鱼能够有效地利用体内储存的脂肪,对饥饿的耐受能力较强.
실험연구료태평양해어(Oncorhynchus spp.)경8-32d기아후대기생장、체조성여혈장생화지표변화적영향.90미초시중약위217 g적태평양해어방양우0.25 m~3적수족상중0-32d,수온위(17.0±2.9)℃.실험분5조,분별위대조조(기아0d)、실험1조(기아8d)、실험2조(기아16d)、실험3조(기아24d)、실험4조(기아32d).매조3개평행,매상6미어.결과표명:기아기간,태평양해어존활솔균위100%,상대체중손실솔여기아시간직선회귀방정위y=0.0086x(R~2=0.9177),정현저적정상관(P<0.05);장계막지방시태평양해어최주요적능최래원,여기아시간정현저적부상관(P<0.05),기직선회귀방정y=-0.0719±4.11(R~2=0.9732);기아초기태평양해어주요소모간장당원화지방、부분장계막지방화소허기육중지방유지생명활동,능모교저:기아후기주요이소모장계막지방、부분기육지방화소량단백질유지생명활동,능모교고;혈장중지방분해매화백단백무현저변화(P<0.05),담동순、감유삼지화고저밀도지단백유현저파동(P<0.05),표명지방대사활약,면역공능미수명현영향.연구표명태평양해어능구유효지이용체내저존적지방,대기아적내수능력교강.
This experiment was conducted to determine the effects of starvation on growth, proximate composition and the biochemical index of plasma of Pacific Salmon (Oncorhynchus spp.). 90 Pacific Salmon with an average weight about 217g were randomly stocked in five-test aquarium (0.25m~3), which was starvation from 0 to 32d. The rearing water temperature was 17.0±2.9℃. 18 fish (six fish from each triplicate aquarium) were sampled at 0, 8, 16, 24 and 32d of each starvation group, respectively. The experiment indicated that no mortality or physical abnormal sign was ob-served in any test group throughout the experiment. Starvation for 32d, the weight was decreased by 7.50% and the relative loss rate of weight was 0.175% on average. At the same time, the relative loss rate of weight and the starvation time had the positive correlation (P<0.05), linear regression equation was y = 0.0086x (R~2 = 0.9177). The lipid of the mesentery was as the main source of the energy, and it had the negative relation with the starvation time, linear regres-sion equation was Y = -0.0719x ± 4.11 (R~2 = 0.9732). Pacific salmon consumed liver fat and hepatic glycogen mainly in the early starvation stage and its energy consumption was relatively low. On later starva. m stage, it mainly consumed mesentery fat, some muscle fat and some proteins for energy source, its energy consumption rose obviously. Lipolytic enzyme (LIPA) and albumin (ALB) did not have significantly difference in the test groups compared with the control group (P > 0.05). The cholesterol (CH), triglyceride (TG) and high-density lipoprotein (HDL) had significant difference among the groups (P < 0.05) and it improved that the metabolic of lipid was active. Pacific salmon had the definitive ability of tolerating starvation relatively better for using the lipid storied in body.