CAJ | 학술논문

目的明确高氧暴露新生大鼠肺组织的肺泡间隔增厚是否由肺泡间隔细胞凋亡抑制引起,进一步研究条碱是否可以通过减少肺泡间隔厚度改善高氧暴露新生大鼠的肺发育.方法建立新生SD大鼠高氧暴露(85%O2)肺损伤模型,将新生大鼠随机分为3组.①新生大鼠每日注射生理盐水并予高氧暴露;②新生大鼠每日注射生理盐水并置正常空气中;③新生大鼠注射茶碱20 mg/(kg·d)并予高氧暴露.并运用TUNEL法检测肺泡间隔中的凋亡细胞,用免疫组织化学技术检测肺泡间隔中的肌成纤维细胞.结果高氧暴露7 d的新生大鼠肺组织肺泡间隔增厚,肺泡间隔细胞凋亡减少,肺泡间隔肌成纤维细胞数量增加;而茶碱可以使高氧暴露后新生大鼠肺组织肺泡间隔细胞变薄,肺泡间隔细胞凋亡增加,肺泡间隔肌成纤维细胞减少.结论茶碱可以通过减少肺泡间隔厚度,减少肺泡间隔内的肌成纤维细胞数量,进而改善高氧暴露新生大鼠的肺发育.增加肺泡间隔细胞凋亡可能是茶碱的作用机制之一.肌成纤维细胞作为一种重要的间质细胞,可能在高氧引起的肺泡间隔细胞凋亡异常及肺泡间隔增厚等变化中起重要作用.
목적 명학고양폭로신생대서폐조직적폐포간격증후시부유폐포간격세포조망억제인기,진일보연구조감시부가이통과감소폐포간격후도개선고양폭로신생대서적폐발육.방법 건립신생SD대서고양폭로(85%O2)폐손상모형,장신생대서수궤분위3조.①신생대서매일주사생리염수병여고양폭로;②신생대서매일주사생리염수병치정상공기중;③신생대서주사다감20 mg/(kg·d)병여고양폭로.병운용TUNEL법검측폐포간격중적조망세포,용면역조직화학기술검측폐포간격중적기성섬유세포.결과 고양폭로7 d적신생대서폐조직폐포간격증후,폐포간격세포조망감소,폐포간격기성섬유세포수량증가;이다감가이사고양폭로후신생대서폐조직폐포간격세포변박,폐포간격세포조망증가,폐포간격기성섬유세포감소.결론 다감가이통과감소폐포간격후도,감소폐포간격내적기성섬유세포수량,진이개선고양폭로신생대서적폐발육.증가폐포간격세포조망가능시다감적작용궤제지일.기성섬유세포작위일충중요적간질세포,가능재고양인기적폐포간격세포조망이상급폐포간격증후등변화중기중요작용.
Objective To investigate the pathogenesis of the thickened distal airspace walls and the potential effects of theophylline through improvement of the alveolarization in the lungs of hyperoxia-exposed rats. Methods Sprague-Dawley neonatal rats were exposed to high concentration of oxygen (85% O2) to study the apoptosis status in hyperoxic lung injury. Pups were divided into three groups (n = 6 in each group). (1) rats injected with saline and exposed to 85% (vol/vol) O2; (2) rats injected with saline and maintained in room air; and (3) rats injected with 8-(p-sulfophenyl)-theophylline dissolved in saline (20 mg/kg·d) and exposed to 85%(vol/vol) O2. A TUNEL assay was used to identify apoptotic cells in the distal airspace walls. Immunochemistry detection was used to estimate the number of myofibroblasts. Results Apoptosís was suppressed and myofibroblasts were increased in the distal airspace walls of lungs in hyperoxia-exposed rats. With subcutaneously injected theophylline, distal airspace wall thickness was decreased, apoptosis was increased, and myofibroblasts were reduced in the distal airspace walls. Conclusions This report demonstrated that apoptosis of cells in distal airspace walls was suppressed by a prolonged hyperoxia exposure.Theophylline could partially thin the distal airspace walls probably by promoting apoptosis, and in this way improved alveolar structure. Myofibroblast, as an prominent mesenchymal cell type, may play an important role in the abnormal apoptotic status during hyperoxia exposure.