CAJ | 학술논문

目的检测白细胞介素-1β(IL-1β)在急性髓系白血病(AML)高白细胞患者外周血中的表达,探讨其在细胞淤滞及向组织浸润中的作用.方法用酶联免疫吸附法(ELISA)测定83例初治AML患者(其中16例为高白细胞白血病患者)血清中IL-1β的含量,比较高白细胞组与非高白细胞组以及浸润组与非浸润组的差别.以40名健康献血员为对照.结果 AML组血清中IL-1β的含量[(88.23±36.30)pg/ml]高于健康对照组[(29.56±15.53)pg/ml],差异有统计学意义(P＜0.01);AML高白细胞组血清中IL-1β含量显著高于非高白细胞组[(136.67±65.68)pg/ml比(69.85±48.35)pg/ml].IL-1β与外周血白细胞呈直线相关关系(r=0.74,P＜0.01).浸润组与非浸润组血清中IL-1β水平为:(111.31±57.35)pg/ml比(79.68±43.42)pg/ml,差异有统计学意义.结论 AML患者血清IL-1 β水平与其外周血白细胞数量有关,在一定程度上可反映疾病的病情,可能参与了白血病细胞组织浸润的机制.
목적 검측백세포개소-1β(IL-1β)재급성수계백혈병(AML)고백세포환자외주혈중적표체,탐토기재세포어체급향조직침윤중적작용.방법 용매련면역흡부법(ELISA)측정83례초치AML환자(기중16례위고백세포백혈병환자)혈청중IL-1β적함량,비교고백세포조여비고백세포조이급침윤조여비침윤조적차별.이40명건강헌혈원위대조.결과 AML조혈청중IL-1β적함량[(88.23±36.30)pg/ml]고우건강대조조[(29.56±15.53)pg/ml],차이유통계학의의(P＜0.01);AML고백세포조혈청중IL-1β함량현저고우비고백세포조[(136.67±65.68)pg/ml비(69.85±48.35)pg/ml].IL-1β여외주혈백세포정직선상관관계(r=0.74,P＜0.01).침윤조여비침윤조혈청중IL-1β수평위:(111.31±57.35)pg/ml비(79.68±43.42)pg/ml,차이유통계학의의.결론 AML환자혈청IL-1 β수평여기외주혈백세포수량유관,재일정정도상가반영질병적병정,가능삼여료백혈병세포조직침윤적궤제.
Objective The interleukin-1β (IL-13) expression in serum of acute myeloid leukemia (AML) patients was evaluated. To explore the significance of IL-1β in leukostasis and tissue infiltration by leukemic cells. Methods ELISA was used to investigate the contents of IL-1β in serum of 83 newly diagnosed with AML which contains 16 hyperleukocytic AML patients, and compared the IL-1β level between the hyperleukocytic AML group and non-hyperleukocytic AML group, the infiltrated group and non-infiltrated group. Results The content of IL-1β in AML serum [(88.23±36.30) pg/ml] was higher than that of in the control group[(29.56±15.53) pg/ml], with significant difference (P ＜0.01). There was a higher level for IL-1β in hyperleukocytic AML group[(136.67±65.68) pg/ml] than in non-hyperleukocytic AML group [(69.85±48.35) pg/ml],and there was a significant difference. IL-13 and peripheral blood cells were in linear correlation (r=0.74, P ＜0.01). There was a higher level for IL-1β in infiltrated group[(111.31 ±57.35) pg/ml] than in the other group [(79.68±43.42) pg/ml], and there was a significant difference. Conclusion The IL-1β may play an important role in leukostasis and tissue infiltration by leukemic cells in AML.