中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2008年
7期
1057-1058,倒插1
,共3页
李书芹%季艳霞%杨志勇%李海燕%张振军%贾军刚%万良刚
李書芹%季豔霞%楊誌勇%李海燕%張振軍%賈軍剛%萬良剛
리서근%계염하%양지용%리해연%장진군%가군강%만량강
乳腺肿瘤%沙立度胺%血管内皮生长因子%细胞系
乳腺腫瘤%沙立度胺%血管內皮生長因子%細胞繫
유선종류%사립도알%혈관내피생장인자%세포계
Breast neoplasm%Thalidomide%Vascular endothelial growth factor%Cell lines
目的 观察沙立度胺对人乳腺癌细胞系MCF-7、MDA-MB-231细胞血管内皮生长因子(VEGF)表达的抑制作用.方法 应用半定量逆转录-聚合酶链反应(RT-PCR)技术,分别观察沙立度胺对MCF-7和MDA-MB-231细胞VEGF表达的抑制作用.结果 沙立度胺在50μg/L明显抑制两株细胞VEGF mRNA的表达,但浓度增高到100μg/L抑制VEGF mRNA表达的作用反而减弱.结论 沙立度胺在一定浓度范围内能抑制乳腺癌细胞VEGF mRNA的表达.
目的 觀察沙立度胺對人乳腺癌細胞繫MCF-7、MDA-MB-231細胞血管內皮生長因子(VEGF)錶達的抑製作用.方法 應用半定量逆轉錄-聚閤酶鏈反應(RT-PCR)技術,分彆觀察沙立度胺對MCF-7和MDA-MB-231細胞VEGF錶達的抑製作用.結果 沙立度胺在50μg/L明顯抑製兩株細胞VEGF mRNA的錶達,但濃度增高到100μg/L抑製VEGF mRNA錶達的作用反而減弱.結論 沙立度胺在一定濃度範圍內能抑製乳腺癌細胞VEGF mRNA的錶達.
목적 관찰사립도알대인유선암세포계MCF-7、MDA-MB-231세포혈관내피생장인자(VEGF)표체적억제작용.방법 응용반정량역전록-취합매련반응(RT-PCR)기술,분별관찰사립도알대MCF-7화MDA-MB-231세포VEGF표체적억제작용.결과 사립도알재50μg/L명현억제량주세포VEGF mRNA적표체,단농도증고도100μg/L억제VEGF mRNA표체적작용반이감약.결론 사립도알재일정농도범위내능억제유선암세포VEGF mRNA적표체.
Objective To assess the effects of thalidomide on VEGF mRNA level in human breast cancer cell lines. Methods Breast cancer cells were treated with thalidomide for 24～72h, RT-PCR was uced to detect the level of VEGF mRNA expression. Results When the concentration of drug is 50μg/L,thalidomide strongly inhibited the level of VEGF mRNA expression. Conclusion Within certain concentration range of thalidomide can inhibit the level of VEGF mRNA expression in breast cancer cell.
