CAJ | 학술논문

目的探讨不同温热条件对HPV感染皮损中E6/E7基因表达的影响.方法采用PCR法确定6例尖锐湿疣标本HPV型别.利用37℃,42℃,45℃不同的温热条件处理置于培养液中的离体尖锐湿疣皮损,采用实时定量PCR法检测HPV-6/11型早期基因E6/E7 mRNA的表达水平.结果 6例标本中.各有1例分别感染HPV-6、HPV-11,4例同时感染HPV-6和HPV-11.E6、E7 mRNA的表达水平均随温度升高而逐渐减少.HPV-6 E6 mRNA的相对表达量分别为37℃(1.00±0.00),42℃(0.61±0.17).45℃(0.27±0.15);HPV-6 E7 mRNA的相对表达量分别为37℃(1.00±0.00),42℃(0.56±0.21),45℃(0.16±0.11);HPV-11 E6 mRNA的相对表达量分别为37℃(1.00±0.00),42℃(0.60±0.22).45℃(0.16±0.08);HPV-11 E7 mRNA相对表达量分别为37℃(1.00±0.00),42℃(0.55±0.15).45℃(0.24±0.06).组间比较差异有统计学意义(P<0.01.结论温热对HPV-6/11型E6和E7基因表达的抑制作用可能是感染消退的机制之一.
목적 탐토불동온열조건대HPV감염피손중E6/E7기인표체적영향.방법 채용PCR법학정6례첨예습우표본HPV형별.이용37℃,42℃,45℃불동적온열조건처리치우배양액중적리체첨예습우피손,채용실시정량PCR법검측HPV-6/11형조기기인E6/E7 mRNA적표체수평.결과 6례표본중.각유1례분별감염HPV-6、HPV-11,4례동시감염HPV-6화HPV-11.E6、E7 mRNA적표체수평균수온도승고이축점감소.HPV-6 E6 mRNA적상대표체량분별위37℃(1.00±0.00),42℃(0.61±0.17).45℃(0.27±0.15);HPV-6 E7 mRNA적상대표체량분별위37℃(1.00±0.00),42℃(0.56±0.21),45℃(0.16±0.11);HPV-11 E6 mRNA적상대표체량분별위37℃(1.00±0.00),42℃(0.60±0.22).45℃(0.16±0.08);HPV-11 E7 mRNA상대표체량분별위37℃(1.00±0.00),42℃(0.55±0.15).45℃(0.24±0.06).조간비교차이유통계학의의(P<0.01.결론 온열대HPV-6/11형E6화E7기인표체적억제작용가능시감염소퇴적궤제지일.
Objective To investigate the effect of hyperthermia on the expression of E6 and E7 genes of human papillomavirus (HPV) type 6 and 11 in HPV-infected human skin. Methods Tissue samples were obtained from the lesions of condyloma accuminatum (CA) in 6 patients after informed consent. Each sample was divided into 4 parts: one was embedded and directly stored at -80 ℃; the other 3 parts were placed in culture medium and the surface of the samples was irradiated for 30 minutes with a thermotherapy apparatus at 37℃, 42 ℃, 45 ℃, respectively, then the samples were taken out and stored at -80 ℃. RNA was extracted from the specimens, real time quantitative PCR (qPCR) was performed to detect the expression of E6 and E7 genes of HPV-6 and -11. Results Of the 6 patients, 2 were infected with HPV-6 and -11 respectively, 4 with both HPV-6 and HPV-11. The expression of E6 and E7 mRNA decreased with the increase in irradiation temperature. The relative mRNA expression levels at 37 ℃, 42 ℃ and 45 ℃ were 1.00 ± 0.00, 0.61 ± 0.17, 0.27 ± 0.15, respectively, for HPV-6 E6 gene, 1.00 ± 0.00, 0.56 ± 0.21, 0.16 ± 0.11 respectively, for HPV-6 E7 gene, 1.00 ± 0.00, 0.60 ± 0.22, 0.16 ± 0.08, respectively, for HPV-I1 E6 gene, 1.00 ± 0.00, 0.55 ± 0.15, 0.24 ± 0.06, respectively, for HPV-11 E7 gene; statistical difference was noted among them between the specimens irradiated at different temperature (all P < 0.01). Conclusion Hyperthermia can remarkably suppress the expression of HPV-6/I 1 E6 and E7 genes, which may be a possible mechanism under the regression of warts induced by local hyperthermia.