CAJ | 학술논문

目的探讨从大鼠的脂肪组织中分离、培养脂肪干细胞(ADSCs)并定向诱导分化为软骨细胞的可行性.方法取Wistar雄性大鼠脂肪组织培养,传代.并用免疫荧光法检测传代ADSCs表面抗原CD29表达.取第3代ADSCs用含有TGF-β1的培养基定向诱导培养,行Ⅱ型胶原免疫组化染色进行检测鉴定.结果显微镜下可见脂肪干细胞的形态特点.并经免疫荧光法进行检测证实.定向诱导ADSCs向软骨细胞分化,观察细胞形态变化,并通过Ⅱ型胶原免疫组化检测,证实向表达Ⅱ型胶原的软骨细胞表型分化的可行性.结论证实大鼠脂肪组织中存在干细胞来源细胞.并且细胞在体外培养条件下生物学性状保持稳定.通过外源性转化生长因子TGF-β定向分化、诱导、培养可表现出软骨细胞的部分特性.证实脂肪干细胞具有向软骨细胞定向分化的能力.
목적 탐토종대서적지방조직중분리、배양지방간세포(ADSCs)병정향유도분화위연골세포적가행성.방법 취Wistar웅성대서지방조직배양,전대.병용면역형광법검측전대ADSCs표면항원CD29표체.취제3대ADSCs용함유TGF-β1적배양기정향유도배양,행Ⅱ형효원면역조화염색진행검측감정.결과 현미경하가견지방간세포적형태특점.병경면역형광법진행검측증실.정향유도ADSCs향연골세포분화,관찰세포형태변화,병통과Ⅱ형효원면역조화검측,증실향표체Ⅱ형효원적연골세포표형분화적가행성.결론 증실대서지방조직중존재간세포래원세포.병차세포재체외배양조건하생물학성상보지은정.통과외원성전화생장인자TGF-β정향분화、유도、배양가표현출연골세포적부분특성.증실지방간세포구유향연골세포정향분화적능력.
Objective To investigate the methods of isolation, cultivation of adipose derived stem cells (ADSCs) from the rats'adipose tissue and the feasibility of inducting differentiation to chondrocyte.Methods The adipose tissue was obtained from the wistar's male rats, and it was isolated, cultivated, and subcultured. The expression of CD29 in the subcultured ADSCs was detected by immunofluorescence. The 3rd generation ADSCs was cultured in the medium contained TGF-β, and the presence was identified by type Ⅱ collagen immunohistochemistry. Results The character of ADSCs'morphology could be observed by microscope, and it can be identified by immunofluorescence. Through the course of inducting the ADSCs to chondrocyte, the feasibility of inducting ADSCs to chondrocyte showed that type Ⅱ collagen was expressed.Conclusion We demonstrated the presence of stem cells in the adipose tissue, and the stability of biological feature in vitro. The chondrocyte could be obtained from the adipose tissue through the committed differentiation, induction, cultivation by exogenous transforming growth factor (TGF-β). The ability of the adipose stem cell differentiating to chondrocyte was also proved.