CAJ | 학술논문

目的观察单壁碳纳米管-聚酰胺树形分子复合物(CNT-PAMAM-D)进入胰腺癌细胞的过程，评价其作为载体的安全性。方法通过超声、搅拌及洗涤等方法制备CNT-PAMAM-D，用原子力显微镜和透射电镜观察其形态。将CNT-PAMAM-D与人胰腺癌细胞BxPC3共同培养12、48、72 h，收集细胞，在透射电镜下观察细胞内CNT-PAMAM-D的分布及细胞超微结构的改变。结果 PAMAM-D与CNT结合之后，树形分子包裹在CNT的表面，形成了20 nm左右大小的纳米复合物颗粒。CNT-PAMAM-D通过细胞吞饮方式被吞入BxPC3细胞，12 h后即大量进入细胞质内，随着时间延长，CNT-PAMAM-D还可进入溶酶体及细胞核中，但胰腺癌细胞的形态及超微结构无显著变化。结论 CNT-PAMAM-D是一种高效、安全的纳米载体。
목적 관찰단벽탄납미관-취선알수형분자복합물(CNT-PAMAM-D)진입이선암세포적과정，평개기작위재체적안전성。방법 통과초성、교반급세조등방법제비CNT-PAMAM-D，용원자력현미경화투사전경관찰기형태。장CNT-PAMAM-D여인이선암세포BxPC3공동배양12、48、72 h，수집세포，재투사전경하관찰세포내CNT-PAMAM-D적분포급세포초미결구적개변。결과 PAMAM-D여CNT결합지후，수형분자포과재CNT적표면，형성료20 nm좌우대소적납미복합물과립。CNT-PAMAM-D통과세포탄음방식피탄입BxPC3세포，12 h후즉대량진입세포질내，수착시간연장，CNT-PAMAM-D환가진입용매체급세포핵중，단이선암세포적형태급초미결구무현저변화。결론 CNT-PAMAM-D시일충고효、안전적납미재체。
Objective To observe the process of polyamidoamine dendrimer modified single walled carbon nanotubes complexes (CNT-PAMAM-D) entering into pancreatic cancer BxPC3 cells, and to evaluate its safety as a vector. Methods The CNT-PAMAM-D were prepared by ultrasound, mixing and rinsing, and the morphology was characterized by atom force microscopy and transmission electron microscope. Then the prepared CNT-PAMAM-D was incubated with human pancreatic cancer cell line BxPC3 cells for 12, 48, 72hours, and then the cells were collected. Distribution of CNT-PAMAM-D in cells and cell ultrastructure was observed by transmission electron microscopy. Results After the combination of PAMAM-D and CNT, the surface of CNT was surrounded by dendrimer and nanocomposite particles with a size of 20 nm was formed. It was showed that CNT-PAMAM-D could be transfected into human pancreatic cancer cells by cell pinocytotic way, and it entered cytoplasm at 12 h. With the extension of the transfecting time, CNT-PAMAM-D could enter lysosomal and nucleus, but the morphology and ultrastructure ocf BxPC 3 cells was not significantly changed. Conclusions The CNT-PAMAM-D is a highly effective and safe nano vector.