中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2010年
12期
933-936
,共4页
顾沈阳%雍永松%王业华%杜拥军
顧瀋暘%雍永鬆%王業華%杜擁軍
고침양%옹영송%왕업화%두옹군
膀胱肿瘤%图像细胞测定%原位杂交,荧光%尿细胞学检查
膀胱腫瘤%圖像細胞測定%原位雜交,熒光%尿細胞學檢查
방광종류%도상세포측정%원위잡교,형광%뇨세포학검사
Urinary bladder neoplasms%Image cytometry%In situ hybridization,fluorescence%Urine cytology
目的 探讨荧光原位杂交法(FISH)和全自动图像细胞仪(ICM)在膀胱尿路上皮癌诊断中的应用.方法 在2008年8月至2009年3月共选取60例患者,包括20例非尿路上皮癌和40例膀胱尿路上皮癌的患者,取患者的尿液作常规尿细胞学检查、FISH和ICM检测.结果 FISH的敏感性显著高于ICM的敏感性(82.5%比62.5%,P＜0.05)和常规尿细胞学的敏感性(82.5%比25.0%,P＜0.05),同时ICM敏感性也高于常规尿细胞学的敏感性(62.5%比25.0%,P＜0.05);FISH、ICM和常规尿细胞学检查的特异性都为100%,三者在特异性方面差异无统计学意义(P＞0.05).FISH、ICM和常规尿脱落细胞学检测的敏感性与病理分期无相关性(P＞0.05),但与分级有相关性(P＜0.05).结论 FISH和ICM在膀胱尿路上皮癌诊断中,其特异性和常规尿细胞学检查一致,但敏感性显著高于常规尿细胞学检查;同时FISH在膀胱尿路上皮癌诊断中的敏感性高于ICM,所以FISH技术更有望成为膀胱尿路上皮癌无创性的诊断和检测手段.
目的 探討熒光原位雜交法(FISH)和全自動圖像細胞儀(ICM)在膀胱尿路上皮癌診斷中的應用.方法 在2008年8月至2009年3月共選取60例患者,包括20例非尿路上皮癌和40例膀胱尿路上皮癌的患者,取患者的尿液作常規尿細胞學檢查、FISH和ICM檢測.結果 FISH的敏感性顯著高于ICM的敏感性(82.5%比62.5%,P＜0.05)和常規尿細胞學的敏感性(82.5%比25.0%,P＜0.05),同時ICM敏感性也高于常規尿細胞學的敏感性(62.5%比25.0%,P＜0.05);FISH、ICM和常規尿細胞學檢查的特異性都為100%,三者在特異性方麵差異無統計學意義(P＞0.05).FISH、ICM和常規尿脫落細胞學檢測的敏感性與病理分期無相關性(P＞0.05),但與分級有相關性(P＜0.05).結論 FISH和ICM在膀胱尿路上皮癌診斷中,其特異性和常規尿細胞學檢查一緻,但敏感性顯著高于常規尿細胞學檢查;同時FISH在膀胱尿路上皮癌診斷中的敏感性高于ICM,所以FISH技術更有望成為膀胱尿路上皮癌無創性的診斷和檢測手段.
목적 탐토형광원위잡교법(FISH)화전자동도상세포의(ICM)재방광뇨로상피암진단중적응용.방법 재2008년8월지2009년3월공선취60례환자,포괄20례비뇨로상피암화40례방광뇨로상피암적환자,취환자적뇨액작상규뇨세포학검사、FISH화ICM검측.결과 FISH적민감성현저고우ICM적민감성(82.5%비62.5%,P＜0.05)화상규뇨세포학적민감성(82.5%비25.0%,P＜0.05),동시ICM민감성야고우상규뇨세포학적민감성(62.5%비25.0%,P＜0.05);FISH、ICM화상규뇨세포학검사적특이성도위100%,삼자재특이성방면차이무통계학의의(P＞0.05).FISH、ICM화상규뇨탈락세포학검측적민감성여병리분기무상관성(P＞0.05),단여분급유상관성(P＜0.05).결론 FISH화ICM재방광뇨로상피암진단중,기특이성화상규뇨세포학검사일치,단민감성현저고우상규뇨세포학검사;동시FISH재방광뇨로상피암진단중적민감성고우ICM,소이FISH기술경유망성위방광뇨로상피암무창성적진단화검측수단.
Objective To discuss the application of automated DNA image cytometry(ICM) and fluorescence in sitn hybridization (FISH) in the diagnosis of urothelial carcinoma of bladder. Methods From August 2008 to March 2009,60 volunteers with informed consent were divided into two groups,40 patients proven as urothehal carcinoma of bladder by pathology and 20 healthy individuals as control.Urine was collected and tested by cytology, ICM and FISH. Results Overall sensitivity of FISH was significantly higher in detection of malignancy than that of ICM(82. 5% vs 62. 5%, P ＜0.05) and that of urine cytology ( 82. 5% vs 25.0%, P ＜ 0. 05 ), while ICM was more sensitive to diagnose urothelial carcinoma of bladder than urine cytology(62. 5% vs 25.0%, P ＜ 0. 05 ). Specificities of urine cytology,ICM and FISH were 100% in diagnosis of urothelial carcinoma of bladder (P ＞ 0. 05 ). Sensitivities of urine cytology, ICM and FISH have no correlation with pathological stage (P ＞ 0.05 ), but have significantcorrelation with grade( P ＜ 0. 05 ). Conclusions ICM and FISH have the same specificity as urine cytology in diagnosis of urothelial carcinoma of bladder, but they have significantly higher sensitivity than urine cytology. FISH has the highest sensitivity among three diagnostic methods. Therefore, FISH may become a newly non-invasive technique for the diagnosis and surveillance of urothelial carcinoma of bladder.
