CAJ | 학술논문

利用长江流域推广品种泗棉3号和优异纤维种质系7235为育种亲本,配置了系统育种和修饰回交聚合育种两套群体.基于来自7235的2个高强纤维主效QTL的分子标记,在上述育种群体中进行了分子标记辅助选择效率研究.高强纤维主效QTLfs-1是利用(7235×TM-1) F2 分离群体,通过集团混合分离法检测到的,它可解释纤维强度表型变异的30%以上.高强纤维主效QTLfs-2最初是利用(HS427-10×TM-1) F2分离群体检测到的,它可解释纤维强度表型变异的12.5%以上.进一步的研究表明,该QTL也位于7235优质系中,但与QTLfs-1非等位.2套育种分离群体的2个高强纤维主效QTL的分子标记辅助选择效果表明:QTLfs-1在不同环境条件下均稳定表达,它对不同遗传背景的育种群体均有显著的选择效果.尽管QTLfs-2的选择效果低于QTLfs-1,它在高世代育种群体中也表现较高的选择效率.利用分子标记辅助选择具有一定遗传距离的QTLfs-1区间,其纤维强度的选择效率将大大增强.通过分子标记对位于不同连锁群上的2个QTL聚合选择,其中选单株的纤维强度显著提高.研究结果为利用分子标记辅助聚合优质QTL提供了成功实例.
이용장강류역추엄품충사면3호화우이섬유충질계7235위육충친본,배치료계통육충화수식회교취합육충량투군체.기우래자7235적2개고강섬유주효QTL적분자표기,재상술육충군체중진행료분자표기보조선택효솔연구.고강섬유주효QTLfs-1시이용(7235×TM-1) F2 분리군체,통과집단혼합분리법검측도적,타가해석섬유강도표형변이적30%이상.고강섬유주효QTLfs-2최초시이용(HS427-10×TM-1) F2분리군체검측도적,타가해석섬유강도표형변이적12.5%이상.진일보적연구표명,해QTL야위우7235우질계중,단여QTLfs-1비등위.2투육충분리군체적2개고강섬유주효QTL적분자표기보조선택효과표명:QTLfs-1재불동배경조건하균은정표체,타대불동유전배경적육충군체균유현저적선택효과.진관QTLfs-2적선택효과저우QTLfs-1,타재고세대육충군체중야표현교고적선택효솔.이용분자표기보조선택구유일정유전거리적QTLfs-1구간,기섬유강도적선택효솔장대대증강.통과분자표기대위우불동련쇄군상적2개QTL취합선택,기중선단주적섬유강도현저제고.연구결과위이용분자표기보조취합우질QTL제공료성공실례.
Based on two major QTLs that control high fiber strength which originated from an elite fiber germplasm line 7235 (Gossypium hiusutum L.),the efficiency of molecular marker-assisted selection (MAS) was investigated using two populations from pedigree selection and modified backcrossing pyramiding developed for the breeding purpose.Simian 3 (SM3),a widely planted variety in the Yangtze River Valley,and 7235 were used as parents to develop the two populations.In the two major QTLs for fiber strength from 7235,QTLfs-1 could explain more than 30% of the phenotypic variation (PV) in the (7235 ×TM-1) F2 population.QTLfs-2 was at first identified in another super quality fiber line HS427-10 from (HS427-10×TM-1) F2 population with 12.5% of PV explanation,which was further also identified in 7235 line but was non-allelic with QTLfs-1.The result of molecular marker-assisted selection for fiber strength showed that the genetic effect of the QTLfs-1 was stable under different environmental conditions,and its molecular marker-assisted selection showed significant selective efficiency among breeding populations with different genetic backgrounds.QTLfs-2 also showed high selective efficiency in advanced generation populations though its effect was a little lower than the former.When QTLfs-1 was selected simultaneously with 2 molecular markers with known genetic distance,the selection efficiency for the fiber strength was greatly increased.The pyramiding for two QTLs that control high fiber strength by MAS greatly improved the selection efficiency for cotton fiber strength.This report provides a successful example of MAS pyramiding for QTL for favorable traits in breeding programs.