CAJ | 학술논문

[目的] 建立蜂胶中氟胺氰菊酯和氟氯苯氰菊酯残留的测定方法.[方法] 以石油醚为溶剂提取样品,采用凝胶渗透色谱法净化样品,采用气相色谱法测定氟胺氰菊酯和氟氯苯氰菊酯残留.[结果] 匀浆提取的提取效果远远好于震荡提取的提取效果.石油醚的提取效果比石油醚-丙酮混合液的提取效果好.与丙酮-石油醚(9∶1)和石油醚-乙酸乙酯(95∶5)相比,石油醚-乙酸乙酯(98∶2)的净化效果最理想.氟胺氰菊酯和氟氯苯氰菊酯的回收率均在69.0%~96.4%,相对标准偏差均在1.27%~7.69%,表明该方法的准确度和精密度均能达到残留分析的要求.氟胺氰菊酯和氟氯苯氰菊酯的检测限均为0.05 mg/kg.[结论] 该方法可以有效监控蜂胶中相应药物的残留,提高国产蜂胶的品质和国际竞争力.
[목적] 건립봉효중불알청국지화불록분청국지잔류적측정방법.[방법] 이석유미위용제제취양품,채용응효삼투색보법정화양품,채용기상색보법측정불알청국지화불록분청국지잔류.[결과] 균장제취적제취효과원원호우진탕제취적제취효과.석유미적제취효과비석유미-병동혼합액적제취효과호.여병동-석유미(9∶1)화석유미-을산을지(95∶5)상비,석유미-을산을지(98∶2)적정화효과최이상.불알청국지화불록분청국지적회수솔균재69.0%~96.4%,상대표준편차균재1.27%~7.69%,표명해방법적준학도화정밀도균능체도잔류분석적요구.불알청국지화불록분청국지적검측한균위0.05 mg/kg.[결론] 해방법가이유효감공봉효중상응약물적잔류,제고국산봉효적품질화국제경쟁력.
[Objective] The aim was to establish the method of determining fluvalinate and cypermethrin residues in propolis. [Method] The samples were extracted with petroleum ether as solvent and purified by gel permeation chromatography. The fluvalinate and cypermethrin residues were determined by gas chromatography. [Result] The extraction effect of homogenination extraction was far better than that of shaking extraction. The extraction effect of petroleum ether was better than that of mixed petroleum ether-acetone solution. Compared with acetone-petroleum ether (9∶1) and petroleum ether-ethyl acetate (95∶5), the purification effect of petroleum ether-ethyl acetate (98∶2) was most perfect. The recoveries of both fluvalinate and cypermethrin were between 69.0% and 96.4% and their relative standard deviations were between 1.27% and 7.69%, showing that both accuracy and precision of this method could meet the requirement for residue analysis. The detection limits of both fluvalinate and cypermethrin were 0.05 mg/kg. [Conclusion] The corresponding drug residues in propolis could be monitored effectively and the quality and international competitive power of China-made propolis could be enhanced by this method.