中国法医学杂志
中國法醫學雜誌
중국법의학잡지
CHINESE JOURNAL OF FORENSIC MEDICINE
2009年
6期
365-367
,共3页
邢浩伟%陈玉川%成建定%高翠莲%罗光华
邢浩偉%陳玉川%成建定%高翠蓮%囉光華
형호위%진옥천%성건정%고취련%라광화
法医病理学%死亡时间推断%DNA含量%腐败尸体%肋软骨%软骨细胞
法醫病理學%死亡時間推斷%DNA含量%腐敗尸體%肋軟骨%軟骨細胞
법의병이학%사망시간추단%DNA함량%부패시체%륵연골%연골세포
forensic pathology%postmortem interval%DNA content%putrefactive cadaver%chondrocytes%costal cartilage
目的 研究大鼠死后肋软骨细胞核DNA含量与晚期死亡时间的相关性.方法 应用改良的Feulgen法结合计算机图像分析技术对死后大鼠35d内肋软骨细胞核DNA含最变化进行观测分析.结果 死后1-28d,细胞核染色逐渐变淡,至35d时已观察不到,死后肋软骨细胞核DNA含量与死亡时间呈直线相关.结论 大鼠肋软骨细胞核DNA含最随死亡时间延长呈线性规律下降.
目的 研究大鼠死後肋軟骨細胞覈DNA含量與晚期死亡時間的相關性.方法 應用改良的Feulgen法結閤計算機圖像分析技術對死後大鼠35d內肋軟骨細胞覈DNA含最變化進行觀測分析.結果 死後1-28d,細胞覈染色逐漸變淡,至35d時已觀察不到,死後肋軟骨細胞覈DNA含量與死亡時間呈直線相關.結論 大鼠肋軟骨細胞覈DNA含最隨死亡時間延長呈線性規律下降.
목적 연구대서사후륵연골세포핵DNA함량여만기사망시간적상관성.방법 응용개량적Feulgen법결합계산궤도상분석기술대사후대서35d내륵연골세포핵DNA함최변화진행관측분석.결과 사후1-28d,세포핵염색축점변담,지35d시이관찰불도,사후륵연골세포핵DNA함량여사망시간정직선상관.결론 대서륵연골세포핵DNA함최수사망시간연장정선성규률하강.
Objective To study the relationship between the DNA content of chondrocytes in the costal cartilage and postmortem interval in putrefactive rat cadavers.Methods Nuclear DNA wag visualized by modified Feulgen's staining method.DNA content of ehondrocytes in the costal cartilage was semi-quantita tively determined by a computerized image analysis system in rats within 35d postmortem.Results Staining intensity of the nuclei was gradually reduced within from 1d to 28d postmortem.The nuclej could not be detected at 35d.The DNA content of chondrocytes decreased time-dependently within 28 days after death as determined semi-quantitatively,which revealed a linear relationship between DNA content and postmortem interval.Conclusion DNA content of chondrocytes in the costal cartilage reduces time-dependently with the extension of postmortem interval.
