CAJ | 학술논문

目的:筛选肺鳞癌淋巴结转移相关的蛋白质. 方法:采用激光捕获显微切割技术(laser capture microdissection,LCM)分别从肺鳞癌原发灶组织和匹配的淋巴结转移灶癌组织中切割纯化鳞癌细胞;应用二维凝胶电泳(two-dimensional electrophoresis,2-DE)分离LCM纯化细胞的蛋白质;图像分析识别差异表达的蛋白质点;基质辅助激光解吸电离飞行时间质谱鉴定差异蛋白质点,Western印迹验证差异蛋白Rho-GDIα在LCM纯化的肺鳞癌原发灶肿瘤细胞(lung primary tumor cells, LPTC)和匹配的淋巴结转移灶肿瘤细胞(lymph node metastatic tumor cells, LNMTC)中的表达.结果:建立了LCM纯化的LPTC和匹配的LNMTC的2-DE图谱,质谱鉴定了22个差异蛋白质,与LPTC相比,8个蛋白质在LNMTC中表达明显增高.结论:22个差异蛋白可能与肺鳞癌淋巴结转移有关,为筛选肺鳞癌转移标志物奠定了基础.
목적:사선폐린암림파결전이상관적단백질. 방법:채용격광포획현미절할기술(laser capture microdissection,LCM)분별종폐린암원발조조직화필배적림파결전이조암조직중절할순화린암세포;응용이유응효전영(two-dimensional electrophoresis,2-DE)분리LCM순화세포적단백질;도상분석식별차이표체적단백질점;기질보조격광해흡전리비행시간질보감정차이단백질점,Western인적험증차이단백Rho-GDIα재LCM순화적폐린암원발조종류세포(lung primary tumor cells, LPTC)화필배적림파결전이조종류세포(lymph node metastatic tumor cells, LNMTC)중적표체.결과:건립료LCM순화적LPTC화필배적LNMTC적2-DE도보,질보감정료22개차이단백질,여LPTC상비,8개단백질재LNMTC중표체명현증고.결론:22개차이단백가능여폐린암림파결전이유관,위사선폐린암전이표지물전정료기출.
Objective To search for lymph node metastasis-associated proteins in human lung squamous carcinoma (hLSC).Methods Laser capture microdissection (LCM) was used to purify the target cells from lung primary tumor and matched lymph node metastatic tumor in hLSC. Two-dimensional gel electrophoresis (2-DE) was performed to separate the total proteins of microdissected tumor cells from lung primary tumor and matched lymph node metastatic tumor. PDQuest software was applied to analyze 2-DE images. Differential protein spots between the two types of tissues were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). The expression of Rho-GDIα, one of the differential proteins, in the microdissected lung primary tumor cells (LPTC) and matched lymph node metastatic tumor cells (LNMTC) was detected by Western blot. Results In the present study, 2-DE patterns of microdissected LPTC and LNMTC were established, and 22 differential proteins in the above two tissues were identified, of which 14 were down-regulated in LNMTC and 8 were up-regulated in LNMTC.Conclusion The 22 differential proteins may play some roles in the process of lymph node metastasis in hLSC, and the data provide new clues for metastasis-associated biomarker screen and mechanism of hLSC.