CAJ | 학술논문

[目的]研究制备壳寡糖的方法.[方法]运用基因工程的方法,将人工构建的含有壳聚糖水解酶基因的质粒载体转入大肠杆菌体内并诱导其表达.[结果]可溶的活性壳聚糖酶在大肠杆菌中的表达量高于300 mg/L.[结论]该重组壳聚糖酶与天然来源的壳聚糖酶具有完全相同的比活力,能专一性切断β-1,4-糖苷键,将壳聚糖降解为不含单糖的壳寡糖.
[목적]연구제비각과당적방법.[방법]운용기인공정적방법,장인공구건적함유각취당수해매기인적질립재체전입대장간균체내병유도기표체.[결과]가용적활성각취당매재대장간균중적표체량고우300 mg/L.[결론]해중조각취당매여천연래원적각취당매구유완전상동적비활력,능전일성절단β-1,4-당감건,장각취당강해위불함단당적각과당.
[Objective]The research aimed to study the preparation of chito-oligosacchaside. [Method]Gene engineering was used to transfer plasmid, which contained the gene of chitosan-hydrolyase, into E.coli. Then the enzyme was induced to express. After purification, chitosan-hydrolyase was used to degrade chitosan. [Result]The amount of the expression of chitosan-hydrolyase in E.coli was higher than 300 mg/L. [Conclusion]The recombinate chitosan-hydrolyase had the same specific activity with the chitosan-hydrolyase from nature. It could specifically break β-1,4 glycosidic bonds and degrade chitosan to chitosan-hydrolyase with no monosaccharide.