国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2011年
1期
7-10
,共4页
巴华杰%徐凯%林子清%罗斌%依伟力%刘冰泉%张文杰
巴華傑%徐凱%林子清%囉斌%依偉力%劉冰泉%張文傑
파화걸%서개%림자청%라빈%의위력%류빙천%장문걸
法医遗传学%Profiler plus试剂盒%STR%亲权鉴定%DNA数据库
法醫遺傳學%Profiler plus試劑盒%STR%親權鑒定%DNA數據庫
법의유전학%Profiler plus시제합%STR%친권감정%DNA수거고
Forensic genetics%Profiler plus kit%Short tandem repeats%Paternity testing%DNA database
目的 探讨Profiler plus试剂盒在DNA数据库建设中应用的适用性以及其9个常染色体短串联重复序列(short tandem repeats,STR)基因座在"标准三联体"亲缘关系DNA数据库比对中认定亲权的危险性.方法 在一杀人案例中,将死者和胎儿的DNA数据在全省DNA数据库中进行亲缘关系比对获得比中记录43条,组成43个假定的"标准三联体"家庭.计算其父权指数(paternity index,PI)值与累计父权相对机会(relative chance of paternity,RCP)值;应用Identiflier试剂盒加做到1 5个常染色体STR基因座,观察其排除情况.结果 在43例假定"三联体"中PI值最低为1 724.318 71138(RCP=99.942 039 69%),最高为55 178.198 764 14(RCP=99.998 187 72%),加做到15个STR基因座后,43例假定"三联体"中每例至少2个基因座出现排除,最多6个基因座出现排除的现象,平均排除基因座数为3.63个.结论 Profiler plus试剂盒不适合用于DNA数据库建设;其9个STR基因座应用到亲缘关系鉴定中是危险的;单纯利用RCP值来认定亲缘关系是不安全的;建议应用16个或更多的基因座建设DNA数据库,并在亲缘关系鉴定时应用16个以上的基因座进行判定.
目的 探討Profiler plus試劑盒在DNA數據庫建設中應用的適用性以及其9箇常染色體短串聯重複序列(short tandem repeats,STR)基因座在"標準三聯體"親緣關繫DNA數據庫比對中認定親權的危險性.方法 在一殺人案例中,將死者和胎兒的DNA數據在全省DNA數據庫中進行親緣關繫比對穫得比中記錄43條,組成43箇假定的"標準三聯體"傢庭.計算其父權指數(paternity index,PI)值與纍計父權相對機會(relative chance of paternity,RCP)值;應用Identiflier試劑盒加做到1 5箇常染色體STR基因座,觀察其排除情況.結果 在43例假定"三聯體"中PI值最低為1 724.318 71138(RCP=99.942 039 69%),最高為55 178.198 764 14(RCP=99.998 187 72%),加做到15箇STR基因座後,43例假定"三聯體"中每例至少2箇基因座齣現排除,最多6箇基因座齣現排除的現象,平均排除基因座數為3.63箇.結論 Profiler plus試劑盒不適閤用于DNA數據庫建設;其9箇STR基因座應用到親緣關繫鑒定中是危險的;單純利用RCP值來認定親緣關繫是不安全的;建議應用16箇或更多的基因座建設DNA數據庫,併在親緣關繫鑒定時應用16箇以上的基因座進行判定.
목적 탐토Profiler plus시제합재DNA수거고건설중응용적괄용성이급기9개상염색체단천련중복서렬(short tandem repeats,STR)기인좌재"표준삼련체"친연관계DNA수거고비대중인정친권적위험성.방법 재일살인안례중,장사자화태인적DNA수거재전성DNA수거고중진행친연관계비대획득비중기록43조,조성43개가정적"표준삼련체"가정.계산기부권지수(paternity index,PI)치여루계부권상대궤회(relative chance of paternity,RCP)치;응용Identiflier시제합가주도1 5개상염색체STR기인좌,관찰기배제정황.결과 재43례가정"삼련체"중PI치최저위1 724.318 71138(RCP=99.942 039 69%),최고위55 178.198 764 14(RCP=99.998 187 72%),가주도15개STR기인좌후,43례가정"삼련체"중매례지소2개기인좌출현배제,최다6개기인좌출현배제적현상,평균배제기인좌수위3.63개.결론 Profiler plus시제합불괄합용우DNA수거고건설;기9개STR기인좌응용도친연관계감정중시위험적;단순이용RCP치래인정친연관계시불안전적;건의응용16개혹경다적기인좌건설DNA수거고,병재친연관계감정시응용16개이상적기인좌진행판정.
Objective To explore the applicability of Profiler plus kit in constructing DNA database and the reliability of 9 short tandem repeats from Profiler plus kit in identifying Mother-Child-Father paternity in DNA database. Methods To collect 43 random persons who are matched with known Decedent(Mother) and embryo( Child )in homicide cases, we constructed 43 fictitious trio families. paternity index ( PI ) and relative chance of paternity (RCP) were computed. We obtained 15 short tandem repeats data with Identiflier kit and studied the situation of exclusion. Results The PI in 43 fictitious trio families is from 1 724. 318 711 38 ( RCP = 99. 942 039 69% ) to 55 178. 198 764 14 ( RCP = 99. 998 187 72% ). The number of exclusion locus is from 2 to 6, the average is 3.63. Conclusions Using Profiler plus kit to construct DNA database is not suitable. Paternity testing with the 9 short tandem repeats locus is not reliable. Confirmation of affinity relationship via using the RCP is not reliable either. It is necessary to establish DNA database through 16 or more loci. We recommend using 16 loci or more in paternity testing.
