CAJ | 학술논문

目的探讨淮南市首起0139霍乱弧菌疫情的发生原因和生物学特性。方法血清学及常规细菌学方法对菌株进行鉴定，聚合酶链式反应(PCR)检测霍乱毒力基因，改良K-B药敏实验。结果 (1)培养分离及血清凝集试验结果，2例患者粪便标本全部阳性，密切接触者6人肛拭子全部阴性；甲鱼塘水样18份，阳性5份；甲鱼产蛋沙场样9份，2份阳性；甲鱼蛋7份，阳性3份；甲鱼排泄物11份，均为阴性。(2)血清凝集试验阳性培养物对头孢哌酮、强力霉素耐药；对氨苄西林、四环素中敏；对氟哌酸、庆大霉素、环丙沙星等敏感。(3)血清凝集试验阳性培养物霍乱毒素(CT)、毒素协同调节菌毛(TCP)全部阳性。结论该起0139霍乱弧菌疫情是因甲鱼养殖塘水污染引起，首例患者分离株与该患者所食甲鱼分离株PFGE带型一致，该菌种对头孢哌酮、强力霉素耐药并携带cta、tcpa毒力基因。
목적 탐토회남시수기0139곽란호균역정적발생원인화생물학특성。방법 혈청학급상규세균학방법대균주진행감정，취합매련식반응(PCR)검측곽란독력기인，개량K-B약민실험。결과 (1)배양분리급혈청응집시험결과，2례환자분편표본전부양성，밀절접촉자6인항식자전부음성；갑어당수양18빈，양성5빈；갑어산단사장양9빈，2빈양성；갑어단7빈，양성3빈；갑어배설물11빈，균위음성。(2)혈청응집시험양성배양물대두포고동、강력매소내약；대안변서림、사배소중민；대불고산、경대매소、배병사성등민감。(3)혈청응집시험양성배양물곽란독소(CT)、독소협동조절균모(TCP)전부양성。결론 해기0139곽란호균역정시인갑어양식당수오염인기，수례환자분리주여해환자소식갑어분리주PFGE대형일치，해균충대두포고동、강력매소내약병휴대cta、tcpa독력기인。
Objective To identify the first cases of cholera in Huainan,0139 epidemic causes and biological properties. Methods Conventional methods of serological and bacteriological methods of the strains were identified by polymerase chain reaction (PCR) to detect cholera virulence genes, susceptibility testing modified KB.Results 1 ,was isolated and serum agglutination results,2 patients were all positive stool samples,6 were in close contact with all the negative rectal swab;turtle pond-like 18,5 were positive;turtle egg in battle were like 9,2 were positive ; turtle eggs 7, the positive three copies; turtle waste 11, were negative. 2, or more positive culture drug sensitivity test of the pioneer B, doxycycline resistant; to ampicillin, tetracycline in sensitive;on norfloxacin, gentamicin, ciprofloxacin sensitive. 3,or more positive cultures gene DNA PCR test results,cholera toxin(CT) ,toxin coregulated pilus (TCP) all were positive. Conclusion From the outbreak of cholera 0139 is water pollution caused by turtle ponds,The trip types of 0139 Vibrio cholera were highly homologous with that isolated from green turtle;the bacteria of the pioneer B, doxycycline resistance and carrying cta, tcpa virulence genes.