CAJ | 학술논문

目的评价乙酰左旋肉碱预处理对低氧低糖诱导PC12细胞凋亡的影响.方法 PC12细胞接种于96孔板中,采用随机数字表法,将细胞随机分为5组(n=6):对照组(C组)、细胞损伤组(Ⅰ组)和不同浓度乙酰左旋肉碱预处理组(A1-3组).C组加入含葡萄糖4.5 g/L的DMEM溶液孵育3h；Ⅰ组加入含葡萄糖0.5 g/L和Na2SO4 3 mmol/L的DMEM溶液孵育3h；A1-3组分别加入0.2、0.4和0.6 mmol/L乙酰左旋肉碱孵育24h,然后加入含葡萄糖0.5 g/L和Na2S2O4 3mmol/L的DMEM溶液孵育3h.采用MTT法检测细胞活力,采用TUNEL法检测细胞凋亡率,测定细胞SOD和ATP酶的活性和MDA含量.结果与C组比较,Ⅰ组细胞活力降低,细胞凋亡率升高,SOD和ATP酶的活性降低,MDA含量增加(P＜0.05),A1-3组细胞活力、细胞凋亡率、SOD和ATP酶的活性和MDA含量差异无统计学意义(P＞0.05)；与Ⅰ组比较,A1-3组细胞活力升高,细胞凋亡率降低,SOD和ATP酶的活性升高,MDA含量下降(P＜ 0.05)；A1-3组间细胞活力、细胞凋亡率、SOD和ATP酶的活性和MDA含量比较差异无统计学意义(P＞0.05).结论乙酰左旋肉碱预处理可通过抑制细胞凋亡,减轻低氧低糖诱导PC12细胞损伤.
목적 평개을선좌선육감예처리대저양저당유도PC12세포조망적영향.방법 PC12세포접충우96공판중,채용수궤수자표법,장세포수궤분위5조(n=6):대조조(C조)、세포손상조(Ⅰ조)화불동농도을선좌선육감예처리조(A1-3조).C조가입함포도당4.5 g/L적DMEM용액부육3h；Ⅰ조가입함포도당0.5 g/L화Na2SO4 3 mmol/L적DMEM용액부육3h；A1-3조분별가입0.2、0.4화0.6 mmol/L을선좌선육감부육24h,연후가입함포도당0.5 g/L화Na2S2O4 3mmol/L적DMEM용액부육3h.채용MTT법검측세포활력,채용TUNEL법검측세포조망솔,측정세포SOD화ATP매적활성화MDA함량.결과 여C조비교,Ⅰ조세포활력강저,세포조망솔승고,SOD화ATP매적활성강저,MDA함량증가(P＜0.05),A1-3조세포활력、세포조망솔、SOD화ATP매적활성화MDA함량차이무통계학의의(P＞0.05)；여Ⅰ조비교,A1-3조세포활력승고,세포조망솔강저,SOD화ATP매적활성승고,MDA함량하강(P＜ 0.05)；A1-3조간세포활력、세포조망솔、SOD화ATP매적활성화MDA함량비교차이무통계학의의(P＞0.05).결론 을선좌선육감예처리가통과억제세포조망,감경저양저당유도PC12세포손상.
Objective To investigate the effect of acetyl-L-carnitine (ALC) preconditioning on the PC12 cell apoptosis induced by oxygen-glucose deprivation.Methods PC12 cells were seeded in 96-well plates and randomly divided into 5 groups ( n =6 each):control group (group C),cell injury group (group Ⅰ) and preconditioning with different concentrations of ALC groups (groups A1-3 ).In group C,the cells were incubated with DMEM liquid culture medium containing glucose 0.5 g/L for 3 h.In groups Ⅰ and A1-3 the cells were incubated with DMEM liquid culture medium containing sodium hydrosulfite (Na2S2O4) 3 mmol/L and glucose 0.5 g/L for 3 h,and in addition the cells were pre-incubated with ALC 0.2,0.4 and 0.6 mmol/L for 24 h in groups A1-3 respectively.Cell viability was evaluated by MTF assay,while the apoptosis in cells was detected using TUNEL.The activities of ATPase and SOD and MDA content were also detected.Results Oxygen-glucose deprivation significantly increased the number of apoptotic cells and the content of MDA,and decreased the cell viability and activities of SOD and ATPase in group Ⅰ compared with group C ( P ＜ 0.05).Preconditioning with ALC significantly increased the cell viability and the activities of SOD and ATPaes,and decreased the number of apoptotic cells and the content of MDA in groups A1-3 compared with group Ⅰ ( P ＜ 0.05).Conclusion ALC preconditioning can attenuate PC12 cell injury induced by oxygen-glucose deprivation through inhibition of apoptosis in cells.