CAJ | 학술논문

目的制备六氟化硫脂质微泡,为靶向微泡的研制奠定基础.方法以二硬脂酰磷脂酰胆碱、聚乙二醇磷脂酰乙醇胺为膜成分,六氟化硫为气体核心,薄膜一超声法制备微泡.以SonoVue作对照,观测自制微泡的形态、粒径、浓度、pH值、渗透压等各项理化参数.在二次谐波模式下观察微泡显影体外水囊及兔肾实质,测量分析增强图像峰值灰度.结果自制微泡和SonoVue均大小均匀,圆形,中空透亮,平均直径分别为2.25μm和2.50 μm,粒径分布分别为0.4～10 μm和0.2～10 μm,90%微泡直径分别控制在6 μm和8 μm以内.初始浓度分别为5 x 108～10 X 108/ml和1 x 108～5 x 108/ml,配置后的稳定性均为6 h.显影水囊灰度值分别为121.67±6.76和122.33±4.53(P>0.05),兔肾造影增强峰灰度分别为72.00±7.21和74.65±10.93(P>0.05).结论脂质微泡制备成功,具有良好的理化特性及显影效果.
목적 제비륙불화류지질미포,위파향미포적연제전정기출.방법 이이경지선린지선담감、취을이순린지선을순알위막성분,륙불화류위기체핵심,박막일초성법제비미포.이SonoVue작대조,관측자제미포적형태、립경、농도、pH치、삼투압등각항이화삼수.재이차해파모식하관찰미포현영체외수낭급토신실질,측량분석증강도상봉치회도.결과 자제미포화SonoVue균대소균균,원형,중공투량,평균직경분별위2.25μm화2.50 μm,립경분포분별위0.4～10 μm화0.2～10 μm,90%미포직경분별공제재6 μm화8 μm이내.초시농도분별위5 x 108～10 X 108/ml화1 x 108～5 x 108/ml,배치후적은정성균위6 h.현영수낭회도치분별위121.67±6.76화122.33±4.53(P>0.05),토신조영증강봉회도분별위72.00±7.21화74.65±10.93(P>0.05).결론 지질미포제비성공,구유량호적이화특성급현영효과.
Objective To prepare phospholipid-based sulfur hexafluoride gas-filled microbubbles, which were designed for targeting microbubbles. Methods The microbubbles composed of DSPC and mPEG2000-PE were produced by film-sonication method,and were compared with SonoVue. In the physical chemistry characteristics studies, the morphology, particle diameter, concentration, pH value and osmotic pressure were investigated. In the echogenicity studies, contrast harmonic imaging technique was used to investigate the enhancement of vitro water sac and normal rabbit kidney parenchyma. Results The lipid microbubbles were well-distributed, round with air holes. The average diameter of self-made microbubbles and SonoVue were 2.25 μm and 2.50μm respectively. The average diameter of self-made microbubbles and SonoVue distributed from 0.4 μm to 10 μm and 0.2 μm to 10 μm,and 90% were under 6 μm and 8 μm respectively,the concentration were 5 x 108～10 x 108/ml and 1 x I08～5 x 108/ml respectively with a stability of 6 hours. In vitro water sac, the gray scale of self-made lipid microbubbles and SonoVue were 121.67±6.76 and 122.33 ± 4.53 respectively( P>0.05). In normal rabbit kidney parenchyma, the peak video intensity of normal rabbit kidney parenchyma of self-made lipid microbubbles and SonoVue were 72.00 ± 7.21 and 74. 65± 10.93 respectively(P>0.05). Conclusions The lipid microbubbles have satisfactory physical chemistry characteristics and echogenicity.